[Zbrafish] HA, FLAG and GFP tags in zebrafish

jasmine.blight from adelaide.edu.au via zbrafish%40net.bio.net (by jasmine.blight from adelaide.edu.au)
Wed Apr 15 00:37:54 EST 2009


Has anyone ever encountered any problems with detecting HA, FLAG or
GFP tags in zebrafish expressing constructs containing these tags?

I have used HRP conjugated anti-HA and FLAG antibodies as well as a
non-conjugated anti-HA mouse antibody and have consistently got the
same non-specific banding.



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