From jerryrhee from gmail.com Sun Aug 2 19:45:43 2009 From: jerryrhee from gmail.com (JERRY Rhee) Date: Mon Aug 3 10:46:03 2009 Subject: [Zbrafish] wide-bore pipets Message-ID: <9fc0b432-6951-4a8c-bd29-84cd197a1624@g31g2000yqc.googlegroups.com> Hi, Does anyone know the company name and catalog number to acquire wide- bore pipets, please? I found references to the Kimble-Chase part number 63A53WT but they only have 63A53 or 63A53P, now. Thank you! Jerry From joep.brinkmann from gmail.com Mon Aug 3 02:46:46 2009 From: joep.brinkmann from gmail.com (Joep Brinkmann) Date: Mon Aug 3 10:46:29 2009 Subject: [Zbrafish] Re: Luciferase Expression Vectors Message-ID: <1b0cf01d0908030046g1640a72dre101e368bae59b79@mail.gmail.com> Kristine, I carried out promoter-reporter experiments in zebrafish embryos and luciferase expression vectors were active as is. I tried pRL-CMV, as well as pRL-TK and pRL-SV40. All of these give high luciferase activity at 2 dpf (or even before), when injected into the single cell. Best wishes, Joep Brinkmann 2009/8/1 > Send Zbrafish mailing list submissions to > zbrafish@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/zbrafish > or, via email, send a message with subject or body 'help' to > zbrafish-request@net.bio.net > > You can reach the person managing the list at > zbrafish-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Zbrafish digest..." > > > Today's Topics: > > 1. RE: Zfish Digest, Vol 50, Issue 6 : How old are these fish? > (Gale Clark) > 2. RE: Z Digest, Vol 50, Issue 7 fish with kinky bodies (Gale Clark) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 31 Jul 2009 13:02:46 -0400 > From: "Gale Clark" > Subject: [Zbrafish] RE: Zfish Digest, Vol 50, Issue 6 : How old are > these fish? > To: , > Message-ID: <66F1C864F5E54F80A335D9573FC92F4A@D2HZVNB1> > Content-Type: text/plain; charset="us-ascii" > > > > Gale Clark > gclark@cape.com > +1 508 776 1468 > -----Original Message----- > From: zbrafish-bounces@oat.bio.indiana.edu > [mailto:zbrafish-bounces@oat.bio.indiana.edu] On Behalf Of > zbrafish-request@oat.bio.indiana.edu > Sent: Thursday, July 30, 2009 1:03 PM > To: zbrafish@magpie.bio.indiana.edu > Subject: Zbrafish Digest, Vol 50, Issue 6 > > Send Zbrafish mailing list submissions to > zbrafish@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/zbrafish > or, via email, send a message with subject or body 'help' to > zbrafish-request@net.bio.net > > You can reach the person managing the list at > zbrafish-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Zbrafish digest..." > > > Today's Topics: > > 1. sick fish??? (yin alessa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 29 Jul 2009 19:06:08 -0700 (PDT) > From: yin alessa > Subject: [Zbrafish] sick fish??? > To: bionet-organisms-zebrafish@moderators.isc.org > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > Dear All, > > I had one tank of fish, the males started to get kinky body and become > very short, whereas all the females look very healthy. Does anybody > know what's going on? > > Thanks! > > > > ------------------------------ > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > > End of Zbrafish Digest, Vol 50, Issue 6 > *************************************** > > > > > > ------------------------------ > > Message: 2 > Date: Fri, 31 Jul 2009 14:45:32 -0400 > From: "Gale Clark" > Subject: [Zbrafish] RE: Z Digest, Vol 50, Issue 7 fish with kinky > bodies > To: > Message-ID: <4F2A8D401E134B91AC14E34BAAFE8EBF@D2HZVNB1> > Content-Type: text/plain; charset="us-ascii" > > How many days old are they? How many of the (approximately 12) fish are > affected? > > > Gale Clark > gclark@cape.com > +1 508 776 1468 > > -----Original Message----- > From: zbrafish-bounces@oat.bio.indiana.edu > [mailto:zbrafish-bounces@oat.bio.indiana.edu] On Behalf Of > zbrafish-request@oat.bio.indiana.edu > Sent: Friday, July 31, 2009 1:04 PM > To: zbrafish@magpie.bio.indiana.edu > Subject: Zbrafish Digest, Vol 50, Issue 7 > > Send Zbrafish mailing list submissions to > zbrafish@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/zbrafish > or, via email, send a message with subject or body 'help' to > zbrafish-request@net.bio.net > > You can reach the person managing the list at > zbrafish-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Zbrafish digest..." > > > Today's Topics: > > 1. Luciferase Expression Vector (Kristine Griffett) > 2. Re: sick fish??? (yin alessa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 30 Jul 2009 15:34:44 -0400 > From: Kristine Griffett > Subject: [Zbrafish] Luciferase Expression Vector > To: zbrafish@magpie.bio.indiana.edu > Message-ID: <95938360-29ED-44CB-B267-1C925EDC5586@mail.usf.edu> > Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes > > Hello All, > > We would like to use a luciferase expression vector to tag cells in > zebrafish embryos and were wondering if anyone has tried to use > vectors commercially available from Promega (pRL-CMV) or New England > BioLabs (pCMV-gLUC)? Can these vectors be used "as-is" or does the > luciferase coding region need to be inserted into a Xenopus or > Zebrafish specific vector for expression of the luciferase in the > embryo? > > Kristine Griffett > University of South Florida > Department of Cell Biology, Microbiology and Molecular Biology > 4202 E Fowler Ave > BSF 218 > Tampa, FL 33620 > > > > ------------------------------ > > Message: 2 > Date: Thu, 30 Jul 2009 12:19:56 -0700 (PDT) > From: yin alessa > Subject: [Zbrafish] Re: sick fish??? > To: bionet-organisms-zebrafish@moderators.isc.org > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1 > > BTW: the fish were just changed from fry food to adult food. There > are about 6 pairs in the tank, definitely not overcrowded. > > > > ------------------------------ > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > > End of Zbrafish Digest, Vol 50, Issue 7 > *************************************** > > > > > > ------------------------------ > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > > End of Zbrafish Digest, Vol 51, Issue 1 > *************************************** > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090803/722f515c/attachment.html From yin.alessa from gmail.com Sun Aug 2 12:42:46 2009 From: yin.alessa from gmail.com (yin alessa) Date: Mon Aug 3 10:46:51 2009 Subject: [Zbrafish] Re: sick fish??? References: Message-ID: <6c588089-d7bd-448e-a8c3-f57763f00cb7@t11g2000prh.googlegroups.com> Thank everybody for responding to my post. My fish are transgenic lines expressing dsred. I raise them the same way as all the other fish we have in the fishroom, but only this batch has the problem: small fish with kinky body. Now I tried to raise the male and females separately and see what happens. However, I also generated other transgenic lines with the same promoter. I found that those fish are also weaker than any other fish I have. They seem to age faster. When I crossed them with other fish, few of them got killed in the crossing tank. They also seem to need longer time to recover after crossing. Has anybody had the experiences like this? Any suggestion is highly appreciated! From claudia_hohn from hotmail.com Tue Aug 4 10:20:35 2009 From: claudia_hohn from hotmail.com (Claudia) Date: Tue Aug 4 10:39:00 2009 Subject: [Zbrafish] Re: sick fish??? References: Message-ID: <6b5b9abf-c5d7-45c3-817c-82c8d2929a49@v36g2000yqv.googlegroups.com> On Aug 2, 12:42?pm, yin alessa wrote: > Thank everybody for responding to my post. ?My fish are transgenic > lines expressing dsred. I raise them the same way as all the other > fish we have in the fishroom, but only this batch has the problem: > small fish with kinky body. Now I tried to raise the male and females > separately and see what happens. > > However, I also generated other transgenic lines with the same > promoter. ?I found that those fish are also weaker than any other fish > I have. They seem to age faster. ?When I crossed them with other fish, > few of them got killed in the crossing tank. They also seem to need > longer time to recover after crossing. ?Has anybody had the > experiences like this? ?Any suggestion is highly appreciated! It could be a problem due to inbreeding. We see a similar trend in our facility where we are trying to inbreed our mutant fish to achieve a homogeneous gene pool much like they do with mice. The problem we are seeing is that after 5-6 generations of mating siblings (no group spawn) the fish have deformations of their jaws and spine and overall the fish do not grow as well and stay skinny they also eat less. From sbanerje from uni-koeln.de Tue Aug 4 11:07:56 2009 From: sbanerje from uni-koeln.de (Sanjita Banerjee) Date: Tue Aug 4 11:10:06 2009 Subject: [Zbrafish] Recipe for 1/3 Zf Ringer Solution Message-ID: <1249402076.4a785cdc3ac2a@webmail.uni-koeln.de> hello, i was trying to find out the recipe for 1/3 zebrafish ringer's solution, since the composition used by our lab seems to be different than that given in the zebrafish book. could anyone please tell me the composition of 1/3 zebrafish ringer's solution that is used for maintaining the embryoes? thank you, sanjita From els.janssens from gmail.com Thu Aug 13 09:06:54 2009 From: els.janssens from gmail.com (Els) Date: Thu Aug 13 10:24:07 2009 Subject: [Zbrafish] Probes Message-ID: Hi, Does anyone have the following zebrafish probes? Ephrin-A5a Ephrin-A5b p53 Slit1a Robo2 Mbx-s Is it possible to receive the plasmids? Greetings, Els Els Janssens PhD-student Research Group Neural Circuit Development and Regeneration Department of Biology, Katholieke Universiteit Leuven (K.U.Leuven) Naamsestraat 61, bus 2464 B-3000 Leuven, Belgium Phone: +32 16 323987 Fax: +32 16 324262 E-mail: els.janssens@bio.kuleuven.be http://bio.kuleuven.be/df/LM/ From castrand from mail.nih.gov Thu Aug 13 14:01:59 2009 From: castrand from mail.nih.gov (Daniel Castranova) Date: Thu Aug 13 14:47:45 2009 Subject: [Zbrafish] Call for Speakers for the Zebrafish Husbandry Workshop at the World Aquaculture Society meeting Message-ID: <6D0CFE42-08B0-45E0-B6A2-7D17B8083A23@mail.nih.gov> CALL FOR SPEAKERS 6th Annual Zebrafish Husbandry Workshop At World Aquaculture Society Meeting Aquaneering, Inc. is pleased to announce that the ZHA (Zebrafish Husbandry Association) has agreed to promote and review a Call for Speakers for a portion of the 6th Annual Zebrafish Husbandry Workshop sponsored by Aquaneering and being held in conjunction with the World Aquaculture Society conference in San Diego March 1 – 5 of 2010. Any Technician, Lab Manager, Veterinarian, Architect, Researcher, Vendor, or other party active in Zebrafish Husbandry is eligible to submit a topic for consideration. Submissions will be reviewed by a panel of ZHA members and those whose topics are thought to be potentially most interesting to attendees of the workshop will be invited to present a talk. Each talk will have a time allowance of 15 – 20 minutes. Please include a brief summary with your topic submittal. Submit Topics (or questions) to: admin@zhaonline.org DEADLINE for Submittal: Friday September 4, 2009 Daniel Castranova Aquatic Specialist Charles River Laboratories - Contractor Unit on Vertebrate Organogenesis Laboratory of Molecular Genetics, NICHD, NIH Building 6B, Room 322, 6 Center Drive Bethesda, MD 20892 (301)594-0904 castrand@mail.nih.gov -------------- next part -------------- Skipped content of type multipart/mixedFrom ankitada from usc.edu Mon Aug 17 18:01:26 2009 From: ankitada from usc.edu (Ankita Das) Date: Tue Aug 18 12:21:54 2009 Subject: [Zbrafish] IP protocol for zebrafish embryos Message-ID: Hi, I was wondering if anyone has a working protocol for doing Immunoprecipitation of proteins from zebrafish and if so, do they mind sharing that with me, Thanks, Ankita -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090817/12a7a28d/attachment.html From tmason from uoregon.edu Mon Aug 24 12:30:56 2009 From: tmason from uoregon.edu (Timothy Mason) Date: Mon Aug 24 12:35:38 2009 Subject: [Zbrafish] survey question: container/condition for fish awaiting genotype results Message-ID: <4A92CE50.50001@uoregon.edu> Hi, I'm gathering information from research labs about the types of containers used to hold fish awaiting genotyping results. For example, a fish that undergoes a tail clip will necessarily need to wait while its genotype is determined via PCR, or, fish that are crossed to produce embryos used for a phenotype ID will need to wait somewhere while the embryos develop the phenotype. Where do you keep the fish? Do you use static water containers? Which manufacturer? How much water? Do you have containers that work with your water system to provide fresh water to the fish while it waits for its result? Here at the UO research facility we have developed a standard procedure that allows researchers to keep fish in a plastic container (ZipLoc) with roughly 32 US ounces (~946 ml) of static water for up to 5 days while it waits for its result. We are reviewing this procedure and are hoping to gather information about other best practices for this type of husbandry procedure. Thanks, in advance, for any help you can offer. -Tim -- Timothy Mason UO Zebrafish Facility Manager Eugene OR 97403 phone: 541-346-4598 http://fish.uoregon.edu From gilbertd from cricket.bio.indiana.edu Mon Aug 24 13:06:50 2009 From: gilbertd from cricket.bio.indiana.edu (Don Gilbert) Date: Mon Aug 24 13:11:28 2009 Subject: [Zbrafish] NIH Guide Notice PUBLISHED Message-ID: <200908241806.n7OI6ox22625@cricket.bio.indiana.edu> [via ZFIN Admin ... ] Revised Resource Sharing Plan Instructions for PAR-08-138 and PAR-08-139, Genetic Screens to Enhance Zebrafish Research and Enhancing Zebrafish Research with Research Tools and Techniques (R01) (NOT-HD-09-019) Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institute of Diabetes and Digestive and Kidney Diseases http://grants.nih.gov/grants/guide/notice-files/NOT-HD-09-019.html Notice Number: NOT-HD-09-019 Key Dates Release Date: August 7, 2009 Issued by Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) (http://www.nichd.nih.gov) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), (http://www2.niddk.nih.gov) Purpose Applicants responding to PAR-08-138 (http://grants.nih.gov/grants/ guide/pa-files/PAR-08-138.html), Genetic Screens to Enhance Zebrafish Research, and/or PAR-08-139 (http://grants.nih.gov/grants/guide/pa- files/PAR-08-139.html), Enhancing Zebrafish Research with Research Tools and Techniques, when preparing their Resource Sharing Plan are strongly encouraged to contact the Zebrafish International Resource Center (ZIRC, http://zfin.org/zirc/home/stckctr.php) to discuss their plans for sharing resources created under their proposed application and to receive a cost estimate for deposition of materials at ZIRC. As per NOT-OD-04-042 (http://grants.nih.gov/grants/guide/notice-files/ NOT-OD-04-042.html), investigators may request funds in their application to defray reasonable costs associated with sharing materials or data or transfer of model organisms and associated data to appropriate repositories. These costs should be considered in developing the budget for the project. For applications with modular budgets, this cost estimate should be included as part of the resource sharing plan. For non-modular applications with detailed budgets, the cost estimate should be justified as part of the requested budget. All other aspects of these FOAs remain the same. Inquiries Questions about this Notice should be directed to: Lorette C. Javois, Ph.D. Eunice Kennedy Shriver National Institute of Child Health and Human Development National Institutes of Health Building 6100, Room 4B01, MSC 7500 Bethesda, Maryland 20892-7500 Phone: 301-496-5541 Email: lj89j@nih.gov (e-mail is the strongly preferred method for inquiries) From claudia_hohn from hotmail.com Tue Aug 25 10:31:06 2009 From: claudia_hohn from hotmail.com (Claudia) Date: Tue Aug 25 10:58:49 2009 Subject: [Zbrafish] Re: survey question: container/condition for fish awaiting genotype results References: Message-ID: <26035a82-e4b2-4fbd-b269-8480034a9c58@t13g2000yqt.googlegroups.com> On Aug 24, 12:30?pm, Timothy Mason wrote: > Hi, > I'm gathering information from research labs about the types of > containers used to hold fish awaiting genotyping results. > For example, a fish that undergoes a tail clip will necessarily need to > wait while its genotype is determined via PCR, or, fish that are crossed > to produce embryos used for a phenotype ID will need to wait somewhere > while the embryos develop the phenotype. > Where do you keep the fish? > Do you use static water containers? Which manufacturer? How much water? > Do you have containers that work with your water system to provide fresh > water to the fish while it waits for its result? > > Here at the UO research facility we have developed a standard procedure > that allows researchers to keep fish in a plastic container (ZipLoc) > with roughly 32 US ounces (~946 ml) of static water for up to 5 days > while it waits for its result. We are reviewing this procedure and are > hoping to gather information about other best practices for this type of > husbandry procedure. > > Thanks, in advance, for any help you can offer. > > -Tim > > -- > Timothy Mason > UO Zebrafish Facility Manager > Eugene OR 97403 > > phone: 541-346-4598http://fish.uoregon.edu Hi Tim, I hold one fish in a 1l plastic beaker with lid until results come in. I cut a hole in the lid to fit a tubing with a pasteur pipette attached for air supply. I also change the water once a day. If I did tail clipping I add fungus eliminator to the static system to avoid infections. Claudia From finchg.ohsu.edu from gmail.com Tue Aug 25 13:13:56 2009 From: finchg.ohsu.edu from gmail.com (finchg@ohsu.edu) Date: Tue Aug 25 13:14:57 2009 Subject: [Zbrafish] Re: survey question: container/condition for fish awaiting genotype results References: Message-ID: <15c2ecaa-cb2a-44df-ae26-2fdc6cca22e3@u20g2000prg.googlegroups.com> On Aug 24, 10:30?am, Timothy Mason wrote: > Hi, > I'm gathering information from research labs about the types of > containers used to hold fish awaiting genotyping results. > For example, a fish that undergoes a tail clip will necessarily need to > wait while its genotype is determined via PCR, or, fish that are crossed > to produce embryos used for a phenotype ID will need to wait somewhere > while the embryos develop the phenotype. > Where do you keep the fish? > Do you use static water containers? Which manufacturer? How much water? > Do you have containers that work with your water system to provide fresh > water to the fish while it waits for its result? > > Here at the UO research facility we have developed a standard procedure > that allows researchers to keep fish in a plastic container (ZipLoc) > with roughly 32 US ounces (~946 ml) of static water for up to 5 days > while it waits for its result. We are reviewing this procedure and are > hoping to gather information about other best practices for this type of > husbandry procedure. > > Thanks, in advance, for any help you can offer. > > -Tim > > -- > Timothy Mason > UO Zebrafish Facility Manager > Eugene OR 97403 > > phone: 541-346-4598http://fish.uoregon.edu Regarding storage of finclipped fish awaiting genotype info: We standardly use a stockpile of ~1L plastic aquaria from our old system. Fish are stored individually. We change the water 1-2 days after fin clip and then every week after that up to 2 weeks. The rational is that the fish will foul the water within the first two days, but without food, a reduced metabolism will dramatically slow the rate at which they require water changes. This seems to work well and we only occasionally lose a fish if we stick to these water changes. Others in the lab isolate finclipped fish in 1L skinny AHAB tanks on the system, but this uses a lot of shelf space so numbers are limited. Niether of these approaches is ideal, on the system takes up too much room and off the system takes a considerable amount of time to do the water changes, especially if doing large numbers. I've been trying to think up some better way to handle finclipped fish storage and thus am very interested in other labs techniques as well. I would like to construct a multi-chamber crossing cage style system where 8 or more fish are stored in a row of linked grated bottom pullouts, the entirety of which are sitting in one large trough of water. This would mean a much more simple and quicker water change. I'm sort of stumped on what materials to construct this out of. Has anyone attempted anything like this? Regarding test-crossed fish awaiting phenotype info: We keep these in their crossing cages without a water change, as we always know the offspring phenotype by the fifth day. Gabe Finch Nicolson Lab Oregon Health and Sciences University 3181 SW Sam Jackson Pk. Rd. MRB816-L474 Phone:(503)494-2928 Fax:(503)494-9612 finchg.ohsu.edu@gmail.com From rburdine from Princeton.EDU Tue Aug 25 14:35:36 2009 From: rburdine from Princeton.EDU (Burdine, Rebecca D) Date: Tue Aug 25 14:39:51 2009 Subject: [Zbrafish] Re: survey question: container/condition for fishawaiting genotype results References: <15c2ecaa-cb2a-44df-ae26-2fdc6cca22e3@u20g2000prg.googlegroups.com> Message-ID: <790DAC09623D0A47ACDB126E3C52D8B60B65DD@MBCLUSTER.pu.win.princeton.edu> We seem to be on the lazy side of this. We use plastic containers with lids that we purchase from FIsher Scientific. We have two sizes, one is 2-3 liters (I think) the other is more like 1/2 a liter. We use the large one to hold pairs we are trying to ID. We usually leave a plastic plant in the container. The smaller ones hold individual fish being evaluated by fin-clip and we do not include a plant. In both cases, we fill the containers about 1/2 to 3/4 full with system water and put the lid on. I was told it is important to have space for air exchange. We don't change the water or do anything special until they go back into the system and we often leave our breeding pairs in the larger containers for up to five days. Fin-clips are turned around in 24 hours. On occasion, we may leave fish in fin clip containers for two days, but this makes me nervous. We don't see a lot of death with this protocol. In general, fish that die were either injured during mating or didn't recover from the Tricaine. Becky --------------------------------------------------- Rebecca D. Burdine, Ph.D. Assistant Professor Dept. of Molecular Biology Princeton University Washington Road Mof 433 Princeton, NJ 08544 Phone: (609) 258-7515 Fax: (609) 258-6730 Email: rburdine@princeton.edu Admin Assistant: Anna Schmedel (609) 258-5028 ________________________________ From: zbrafish-bounces@oat.bio.indiana.edu on behalf of finchg@ohsu.edu Sent: Tue 8/25/2009 2:13 PM To: bionet-organisms-zebrafish@moderators.isc.org Subject: [Zbrafish] Re: survey question: container/condition for fishawaiting genotype results On Aug 24, 10:30 am, Timothy Mason wrote: > Hi, > I'm gathering information from research labs about the types of > containers used to hold fish awaiting genotyping results. > For example, a fish that undergoes a tail clip will necessarily need to > wait while its genotype is determined via PCR, or, fish that are crossed > to produce embryos used for a phenotype ID will need to wait somewhere > while the embryos develop the phenotype. > Where do you keep the fish? > Do you use static water containers? Which manufacturer? How much water? > Do you have containers that work with your water system to provide fresh > water to the fish while it waits for its result? > > Here at the UO research facility we have developed a standard procedure > that allows researchers to keep fish in a plastic container (ZipLoc) > with roughly 32 US ounces (~946 ml) of static water for up to 5 days > while it waits for its result. We are reviewing this procedure and are > hoping to gather information about other best practices for this type of > husbandry procedure. > > Thanks, in advance, for any help you can offer. > > -Tim > > -- > Timothy Mason > UO Zebrafish Facility Manager > Eugene OR 97403 > > phone: 541-346-4598http://fish.uoregon.edu Regarding storage of finclipped fish awaiting genotype info: We standardly use a stockpile of ~1L plastic aquaria from our old system. Fish are stored individually. We change the water 1-2 days after fin clip and then every week after that up to 2 weeks. The rational is that the fish will foul the water within the first two days, but without food, a reduced metabolism will dramatically slow the rate at which they require water changes. This seems to work well and we only occasionally lose a fish if we stick to these water changes. Others in the lab isolate finclipped fish in 1L skinny AHAB tanks on the system, but this uses a lot of shelf space so numbers are limited. Niether of these approaches is ideal, on the system takes up too much room and off the system takes a considerable amount of time to do the water changes, especially if doing large numbers. I've been trying to think up some better way to handle finclipped fish storage and thus am very interested in other labs techniques as well. I would like to construct a multi-chamber crossing cage style system where 8 or more fish are stored in a row of linked grated bottom pullouts, the entirety of which are sitting in one large trough of water. This would mean a much more simple and quicker water change. I'm sort of stumped on what materials to construct this out of. Has anyone attempted anything like this? Regarding test-crossed fish awaiting phenotype info: We keep these in their crossing cages without a water change, as we always know the offspring phenotype by the fifth day. Gabe Finch Nicolson Lab Oregon Health and Sciences University 3181 SW Sam Jackson Pk. Rd. MRB816-L474 Phone:(503)494-2928 Fax:(503)494-9612 finchg.ohsu.edu@gmail.com _______________________________________________ Zbrafish mailing list Zbrafish@net.bio.net http://www.bio.net/biomail/listinfo/zbrafish -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090825/58f09938/attachment.html From amp from stowers.org Tue Aug 25 22:43:03 2009 From: amp from stowers.org (Adam Petrie) Date: Wed Aug 26 10:52:20 2009 Subject: [Zbrafish] Re: survey question: container/condition for fish awaiting genotype results References: Message-ID: <6d118779-45f3-4ce6-97c2-e3d1a99cfef8@q5g2000yqh.googlegroups.com> On Aug 24, 12:30?pm, Timothy Mason wrote: > Hi, > I'm gathering information from research labs about the types of > containers used to hold fish awaiting genotyping results. > For example, a fish that undergoes a tail clip will necessarily need to > wait while its genotype is determined via PCR, or, fish that are crossed > to produce embryos used for a phenotype ID will need to wait somewhere > while the embryos develop the phenotype. > Where do you keep the fish? > Do you use static water containers? Which manufacturer? How much water? > Do you have containers that work with your water system to provide fresh > water to the fish while it waits for its result? > > Here at the UO research facility we have developed a standard procedure > that allows researchers to keep fish in a plastic container (ZipLoc) > with roughly 32 US ounces (~946 ml) of static water for up to 5 days > while it waits for its result. We are reviewing this procedure and are > hoping to gather information about other best practices for this type of > husbandry procedure. > > Thanks, in advance, for any help you can offer. > > -Tim > > -- > Timothy Mason > UO Zebrafish Facility Manager > Eugene OR 97403 > > phone: 541-346-4598http://fish.uoregon.edu Most of the time, for holding fish pending ID, the fish are kept in breeder cages made by Aquatic Habitats. Depending on which room the fish are housed, it will either be a 2L breeder cage that is filled about half-full with system water or a 1L breeder cage filled nearly to the top. We may also keep two fish in the cages. If so, both fish are from the same breeding/fin clipping, they are opposite sexes, and a divider is used to keep them separate. If the fish were spawned in the cages, we will reuse those cages for the same fish after rinsing them out with system water. The fish are fed live, freshly hatched Artemia the day they are placed in the cages and once at mid-day thereafter. The water is exchanged every other day until the fish are moved back to a fish system. For an extended period, the cages are replaced and disinfected weekly. Occasionally, a fish we need to hold separate is already being housed individually (or with a fish of the opposite sex) in a system tank. In this case, the fish is returned to its system tank and feeding is as normal. Thanks, Adam Petrie Laboratory Supervisor, Aquatics Stowers Institute for Medical Research From cdietz.hunter from gmail.com Sun Aug 30 11:32:38 2009 From: cdietz.hunter from gmail.com (Caroline) Date: Mon Aug 31 10:31:25 2009 Subject: [Zbrafish] Question about Personnel to Rack/Tank Ratio Message-ID: Hello All, I'm the Zebrafish Technician for a facility with 18 racks (~1100 tanks), and 3 more are scheduled to be installed tomorrow ( for a total of 4 separate recirculating systems). Currently, I'm the only staff member who cares for the fish and facility, and I'm quite concerned that I will not be able to keep up with the workload. Since aquatic model organisms aren't as strictly regulated or as well- established as the murine species, I've not been able to find any information regarding the "typical" job description of the Zebrafish Tech, how other institutions delegate fish facilities duties, and how many people they employ to cover these tasks. So, I was hoping to get some feedback on this matter from other members of the zebrafish community for a means of comparison, and hopefully some information to take to my supervisors. Thanks so much Caroline From khelde from fhcrc.org Mon Aug 31 13:17:49 2009 From: khelde from fhcrc.org (khelde@fhcrc.org) Date: Mon Aug 31 13:27:47 2009 Subject: [Zbrafish] Re: survey question: container/condition for fish awaiting genotype results Message-ID: <20090831111749.x9wdfdcls8440oso@webmail.fhcrc.org> On Aug 24, 12:30 pm, Timothy Mason wrote: > Hi, > I'm gathering information from research labs about the types of > containers used to hold fish awaiting genotyping results. > For example, a fish that undergoes a tail clip will necessarily need to > wait while its genotype is determined via PCR, or, fish that are crossed > to produce embryos used for a phenotype ID will need to wait somewhere > while the embryos develop the phenotype. > Where do you keep the fish? > Do you use static water containers? Which manufacturer? How much water? > Do you have containers that work with your water system to provide fresh > water to the fish while it waits for its result? > > Here at the UO research facility we have developed a standard procedure > that allows researchers to keep fish in a plastic container (ZipLoc) > with roughly 32 US ounces (~946 ml) of static water for up to 5 days > while it waits for its result. We are reviewing this procedure and are > hoping to gather information about other best practices for this type of > husbandry procedure. > > Thanks, in advance, for any help you can offer. > > -Tim > > -- > Timothy Mason > UO Zebrafish Facility Manager > Eugene OR 97403 > > phone: 541-346-4598http://fish.uoregon.edu Hi Tim here in the Moens Lab in Seattle we use small plastic tupperware containers. They hold 500ml if completely full so we put 300-400ml in them. They have 5 holes cut in the lids. They stack on the bench such that we have 8 columns wide and 6 columns high (1/2 of a 96-well plate). To fill 48 "tanklets" we make up 20 liters of system water with AmmoLock (2.8ml/20L; Aquarium Pharmaceuticals) and Stress Coat+ Fish and Tap Water conditioner (5.3ml/20L). We leave the fish in the tanks, without changing the water, from one to 5 days. When finished, we put the tanklets through a bleach process and then send them down to glassware for a final washing (water only). Kathryn Helde Moens Lab