[Zbrafish] Re: survey question: container/condition for
fishawaiting genotype results
Burdine, Rebecca D
(by rburdine from Princeton.EDU)
Tue Aug 25 14:35:36 EST 2009
We seem to be on the lazy side of this.
We use plastic containers with lids that we purchase from FIsher Scientific. We have two sizes, one is 2-3 liters (I think) the other is more like 1/2 a liter.
We use the large one to hold pairs we are trying to ID. We usually leave a plastic plant in the container. The smaller ones hold individual fish being evaluated by fin-clip and we do not include a plant.
In both cases, we fill the containers about 1/2 to 3/4 full with system water and put the lid on. I was told it is important to have space for air exchange.
We don't change the water or do anything special until they go back into the system and we often leave our breeding pairs in the larger containers for up to five days. Fin-clips are turned around in 24 hours. On occasion, we may leave fish in fin clip containers for two days, but this makes me nervous.
We don't see a lot of death with this protocol. In general, fish that die were either injured during mating or didn't recover from the Tricaine.
Rebecca D. Burdine, Ph.D.
Dept. of Molecular Biology
Washington Road Mof 433
Princeton, NJ 08544
Phone: (609) 258-7515
Fax: (609) 258-6730
Email: rburdine from princeton.edu
Admin Assistant: Anna Schmedel (609) 258-5028
From: zbrafish-bounces from oat.bio.indiana.edu on behalf of finchg from ohsu.edu
Sent: Tue 8/25/2009 2:13 PM
To: bionet-organisms-zebrafish from moderators.isc.org
Subject: [Zbrafish] Re: survey question: container/condition for fishawaiting genotype results
On Aug 24, 10:30 am, Timothy Mason <tma... from uoregon.edu> wrote:
> I'm gathering information from research labs about the types of
> containers used to hold fish awaiting genotyping results.
> For example, a fish that undergoes a tail clip will necessarily need to
> wait while its genotype is determined via PCR, or, fish that are crossed
> to produce embryos used for a phenotype ID will need to wait somewhere
> while the embryos develop the phenotype.
> Where do you keep the fish?
> Do you use static water containers? Which manufacturer? How much water?
> Do you have containers that work with your water system to provide fresh
> water to the fish while it waits for its result?
> Here at the UO research facility we have developed a standard procedure
> that allows researchers to keep fish in a plastic container (ZipLoc)
> with roughly 32 US ounces (~946 ml) of static water for up to 5 days
> while it waits for its result. We are reviewing this procedure and are
> hoping to gather information about other best practices for this type of
> husbandry procedure.
> Thanks, in advance, for any help you can offer.
> Timothy Mason
> UO Zebrafish Facility Manager
> Eugene OR 97403
> phone: 541-346-4598http://fish.uoregon.edu <http://fish.uoregon.edu/>
Regarding storage of finclipped fish awaiting genotype info:
We standardly use a stockpile of ~1L plastic aquaria from our old
system. Fish are stored individually. We change the water 1-2 days
after fin clip and then every week after that up to 2 weeks. The
rational is that the fish will foul the water within the first two
days, but without food, a reduced metabolism will dramatically slow
the rate at which they require water changes. This seems to work well
and we only occasionally lose a fish if we stick to these water
Others in the lab isolate finclipped fish in 1L skinny AHAB tanks
on the system, but this uses a lot of shelf space so numbers are
Niether of these approaches is ideal, on the system takes up too
much room and off the system takes a considerable amount of time to do
the water changes, especially if doing large numbers. I've been
trying to think up some better way to handle finclipped fish storage
and thus am very interested in other labs techniques as well. I would
like to construct a multi-chamber crossing cage style system where 8
or more fish are stored in a row of linked grated bottom pullouts, the
entirety of which are sitting in one large trough of water. This
would mean a much more simple and quicker water change. I'm sort of
stumped on what materials to construct this out of. Has anyone
attempted anything like this?
Regarding test-crossed fish awaiting phenotype info:
We keep these in their crossing cages without a water change, as we
always know the offspring phenotype by the fifth day.
Oregon Health and Sciences University
3181 SW Sam Jackson Pk. Rd. MRB816-L474
finchg.ohsu.edu from gmail.com
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