[Zbrafish] Re: making gels for embryo injections

Marie Winandy via zbrafish%40net.bio.net (by mwinandy from ulg.ac.be)
Mon Feb 2 03:51:50 EST 2009


Dear Jonathan, 

We don't use anymore those gels, but put the eggs next to a glass microscope slide, in a dry petri dish, taking away all the water. The eggs are maintained by the slide while you injcet them. After injection you just have to rinse with a wash bottle filled with egg water and collect the eggs in a new, clean petri dish. 

Good luck!

M. Winandy
Marie Winandy, PhD
Université de Liège 
GIGA B34 -  Zebrafish Platform
Avenue de l'Hôpital 1
B-4000 Liège - Sart Tilman 
Belgique
Tél: +32.4.366.99.71  +32.4.366.33.38  +32.476.97.25.33
Fax: +32.4.366.41.98
email : mwinandy from ulg.ac.be or zebrafish.giga from ulg.ac.be 
 http://www.giga.ulg.ac.be/extranet/services.htm




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