[Zbrafish] breeding problems in winter??

Lawrence, Christian via zbrafish%40net.bio.net (by Christian.Lawrence from childrens.harvard.edu)
Tue Feb 3 12:39:52 EST 2009

There should be no seasonal dependency of reproduction in controlled conditions.

If your females don't have "big bellies", they won't breed. You've got to get their bellies big again, so to speak - if you can.

It is likely that they have become senescent because of poor diet and/or static social conditions. Feed them a mixture of a live diet and processed feeds and mix and match holding conditions as much as possible.  Sex segregation is generally unnecessary.  They will still spawn when set up in pairs and this will help keep them cycling if you don't regularly breed them.

Long pauses between events are also not necessarily helpful.  Although you will find it in various places in the "literature" that zebrafish females need a resting period of up to 2 weeks between spawning events, this is not the case if they are in top reproductive condition.  

Christian Lawrence
Aquatic Resources Program
Children's Hospital Boston
320 Longwood Avenue
Boston, MA 02115
617.919.2738 (office)
617.730.0836 (fax)
christian.lawrence from childrens.harvard.edu
-----Original Message-----
From: zbrafish-bounces from oat.bio.indiana.edu [mailto:zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of Anne Streit
Sent: Tuesday, February 03, 2009 10:40 AM
To: bionet-organisms-zebrafish from moderators.isc.org
Subject: [Zbrafish] breeding problems in winter??

Hallo everybody!

IŽm working with zebrafish since octobre last year. We keep them in two large 50 litre tanks, male and females
separated as good as differenciation was possible ;-)
I use Tecniplast breeding boxes for pairing. The first pairings in octobre/november/december worked well.
Unfortunately in the new year the fish have not layed eggs up to now, although IŽve tried several times to pair them.
I also made long pauses to avoid stress for the females.
The females do not have very round bellys :-(

Does anyone know if there is a seasonal dependency? I pair them off-system, means at room temperature and set up pairs
in the afternoon for the next morning.

I would be very grateful for all advices concerning breeding! :-)

Thanks in advance

best regards Anne 
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