[Zbrafish] (no subject)

[April]

Hi Tess,
Here is my recommendation for containing Coleps and/or any other  
unwanted organisms.

1.  Bleach all of your eggs as soon as possible.  If needed, you can  
reference the egg bleaching protocol found in The Zebrafish Book,  
http://zfin.org/zf_info/zfbook/chapt3/3.2.html or on our website,  
http://zebrafish.org/zirc/documents/protocols.php#Egg%20Bleaching.
2.  Be sure to remove ALL dead/damaged eggs before bleaching.
3.  After bleaching, keep your embryos in embryo media until they  
have inflated their swim bladders and are swimming.  Zebrafish larvae  
are the most susceptible when they have hatched, but not yet inflated  
their swim bladders.  Once the larvae are able to swim properly, they  
can fend off most organisms.

If you haven't already positively identified the organisms and would  
like to, I recommend putting a few of these critters on a slide and  
look at them under a compound microscope.  Coleps are quite small and  
move in an intense twirling motion.  They have plates that cover  
their exterior and make them look like hand grenades.  As you  
mentioned, they are voracious and can consume an entire embryo in 45  
minutes.

For future reference, I recommend obtaining a good protozoa picture  
book/guide.  We have 2 older books that are quite helpful, however  
you might be able to find some newer better books with even better  
pictures and information.

- Patterson, D.J. (1996).  Free-Living Freshwater Protozoa (A Colour  
Guide).  John Wiley & Sons Inc., New York, New York.
- Berk, S. G.; Gunderson, J. H (1993). Wastewater Organisms (A Color  
Atlas).  CRC Press LLC.

If you continue to have problems, please let me know.

Very best,

April

On Feb 9, 2009, at 4:17 PM, Fresquez, Theresa, DCA wrote:

> Hello,
> Does anyone know how to get rid of coleps? They are eating my  
> zebrafish babies.
> Thanks,
> Tess
>
>
>
>
>
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-----------------------
April R. Freeman (Mazanec)
ZIRC Manager
Zebrafish Int.'l Resource Center
5274 University of Oregon
Eugene, OR 97403-5274

Phone: (541) 346-6028 ext. 24
Fax: (541) 346-6151


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