[Zbrafish] number of cells of ZF 3-5d old embryos

MILKA via zbrafish%40net.bio.net (by milka.sarris from gmail.com)
Fri Feb 13 07:29:12 EST 2009


Would anyone have an idea of how many total cells can be extracted
from 3,4 or 5 d old ZF embryos with trypsin/EDTA-mediated
dissociation? I am trying to prepare cell suspensions from ZF embryos
and would like to have an idea of how many cells/embryo are possible
to extract at the different developmental stages. Any insight would be
very much appreciated!

Many thanks,


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