[Zbrafish] cold weather shipping
(by Christian.Lawrence from childrens.harvard.edu)
Tue Jan 20 14:23:29 EST 2009
It's probably worth mentioning that as long as the eggs are properly packed (bleached, 1 dpf at ~ 1 embryo/ml in sealed conical tubes or tissue culture flasks wrapped in bubble wrap) inside sealed thick-walled styrofoam containers, no heating packs are necessary IF the fish are received within 24-36 hours after packing. Beyond this, it can get a bit dicey. But we routinely (on a nearly weekly basis) ship domestically without heat packs during cold weather months with no reported problems. The embryos are remarkably tolerant of temperature swings. This isn't too surprising given that zebrafish are among the most eurythermal fish species on record.
A useful little study would be to track temperatures inside sealed packing containers shipped to various locations at different times of year. Perhaps ZIRC has this kind of data?
Aquatic Resources Program
Children's Hospital Boston
320 Longwood Avenue
Boston, MA 02115
christian.lawrence from childrens.harvard.edu
On 1/16/09 1:06 PM, "Steven Waldron" <waldrons from u.washington.edu> wrote:
You'll need to pack the fish in a small thick-walled styrofoam shipping
container nestled within a cardboard box filled with packing peanuts. It
is essential to pack the styrofoam peanut layer with a few heat packs-
the kind used as hand-warmers for cold-weather sports will do. If the
fish are going really long-distance you may have to invest in heat packs
that are specifically designed for shipping livestock and maintain heat
for a longer duration. You can find these at aquabid.com.
p.s. make sure the heat packs do not come into direct contact with the
container holding the fish...ironically, direct contact with the heat
packs could fry your fish even if the outside temperature is frigid.
Judy Bennett wrote:
> Does anyone have a reliable protocol for (overnight) shipping adult
> fish and/or embryos in these frigid temps?
> Judy Bennett
> UMass, Amherst
> Zbrafish mailing list
> Zbrafish from net.bio.net
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