[Zbrafish] GFP and in situ
(by chi-bin.chien from neuro.utah.edu)
Wed Jan 21 17:56:34 EST 2009
No, I don't think this is possible. GFP fluorescence is delicate, in
situs are harsh. However, GFP antigenicity can survive quite well,
and we routinely antibody stain for GFP after in situs.
On Aug 1, 2008, at 11:46 AM, Weibin Zhou wrote:
> I am wondering whether it is possible to preserve GFP fluorescence
> after in situ hybridization (whole-mount or sectioned). If you
> happen to have a protocol that works, please let me know. Thank you
> very much.
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