From Christian.Lawrence from childrens.harvard.edu Thu Jul 9 13:02:22 2009 From: Christian.Lawrence from childrens.harvard.edu (Lawrence, Christian) Date: Thu Jul 9 13:03:35 2009 Subject: [Zbrafish] FW: Reminder: Zebrafish Husbandry Association Meeting - Tuesday July 14th In-Reply-To: Message-ID: Hello All: Please see forwarded meeting announcement below. This will be broadcasted over the web, so you don't have to be in Boston to attend. Contact Marie Ortega (marie.ortega@schepens.harvard.edu) for details. Chris Christian Lawrence Aquatic Resources Program Children's Hospital Boston 320 Longwood Avenue Boston, MA 02115 617.919.2738 617.730.0836 (fax) christian.lawrence@childrens.harvard.edu ------ Forwarded Message From: MA Ortega Date: Thu, 9 Jul 2009 12:52:17 -0400 To: Conversation: Reminder: ZHA Meeting this Tuesday July 14th Subject: Reminder: ZHA Meeting this Tuesday July 14th ZHA Reminder, If you have not RSVP already - please do so by tomorrow - Friday July 10th. Please let us know if you will be present or would like to login via the web. Zebrafish Husbandry Association July 14th, 2009 Meeting 12:00 pm EST "Evaluation of Various Live Feeds and Feeding Regimes on Growth and Survival of Larval Zebrafish" presented by Mr. Isaac Adatto Lead Research Technologist Zon Laboratory and Aquatic Resources Program Children's Hospital, Boston Karp Research Building 8th Floor Conference Room Directions: http://www.childrenshospital.org/patientsfamilies/Site1393/mainpageS1393P87.html This talk will be broadcasted via the web. By the end of this week we will be emailing the login instructions for those of you that RSVP. If you plan on attending in person, please let us know as well because we need to provide your name at the security desk. Thanks. Marie RSVP to Marie Ortega marie.ortega@schepens.harvard.edu ------ End of Forwarded Message From zfinadmin from gmail.com Fri Jul 10 11:50:54 2009 From: zfinadmin from gmail.com (ZFIN Admin) Date: Fri Jul 10 11:52:32 2009 Subject: [Zbrafish] The 4th Asia-Oceania zebrafish meeting - Registration is now open Message-ID: <746276dc-e44c-4bfa-9f3a-3cdd84ea5bb9@p28g2000vbn.googlegroups.com> http://www.cnu.ac.kr/~zebrafish/ From xiaw from umbi.umd.edu Tue Jul 21 15:24:54 2009 From: xiaw from umbi.umd.edu (Wei Xia) Date: Wed Jul 22 11:11:17 2009 Subject: [Zbrafish] Anybody has zebrafish GnRH3 antibody? Message-ID: <2DCEAB95-4C96-4DFB-B0A6-9BA34B494744@umbi.umd.edu> Sorry for the bothering! I urgently need it. It can be against the decapeptide or the gap protein and we will pay the shipping and handling fees. Thanks a lot for your help in advance! Wei From aquagopal from gmail.com Sun Jul 26 08:14:01 2009 From: aquagopal from gmail.com (A. Gopalakrishnan) Date: Mon Jul 27 11:52:36 2009 Subject: [Zbrafish] monodon rough shell disease Message-ID: Dear sir, Please find the weblink of the news letter, *Seshaiyana*, which carries our article titled " Rough-shell disease of *Penaeus monodon.* It may be of useful to you. *http://www.casmbenvis.nic.in/pdf/seshaiyana16(3)2008.pdf* This is for your kind information*.* ** *Regards* ** *Gopalakrishnan* -- __________________________________ Ayyaru GOPALAKRISHNAN, M.Sc., M.Sc.,M.A.,Ph.D., Lecturer (Coastal Aquaculture & Livelihood) Centre of Advanced Study in Marine Biology Annamalai University, Parangipettai 608 502 Tamil Nadu, India Mobile:09443537538 Email: ag_krishnan@yahoo.com aquagopal@gmail.com -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090726/84387d52/attachment.html From jason.cockington from gmail.com Mon Jul 27 21:15:32 2009 From: jason.cockington from gmail.com (Leviathan) Date: Tue Jul 28 11:28:04 2009 Subject: [Zbrafish] Micropipette Holder Message-ID: <0cbd8165-8f23-44f9-9c03-b7734cf55562@y10g2000prg.googlegroups.com> Hi All, i have been having some grief trying to identify the manufacturer of our micropipette holders. I am hoping someone on here might recognize it and be able to tell me who makes them? http://www.adelaide.edu.au/mbs/zebrafish/images/Micropipette_holder_exploded.jpg many thanks, jason From yin.alessa from gmail.com Wed Jul 29 21:06:08 2009 From: yin.alessa from gmail.com (yin alessa) Date: Thu Jul 30 10:50:40 2009 Subject: [Zbrafish] sick fish??? Message-ID: Dear All, I had one tank of fish, the males started to get kinky body and become very short, whereas all the females look very healthy. Does anybody know what's going on? Thanks! From kgriffet from mail.usf.edu Thu Jul 30 14:34:44 2009 From: kgriffet from mail.usf.edu (Kristine Griffett) Date: Thu Jul 30 15:45:02 2009 Subject: [Zbrafish] Luciferase Expression Vector Message-ID: <95938360-29ED-44CB-B267-1C925EDC5586@mail.usf.edu> Hello All, We would like to use a luciferase expression vector to tag cells in zebrafish embryos and were wondering if anyone has tried to use vectors commercially available from Promega (pRL-CMV) or New England BioLabs (pCMV-gLUC)? Can these vectors be used "as-is" or does the luciferase coding region need to be inserted into a Xenopus or Zebrafish specific vector for expression of the luciferase in the embryo? Kristine Griffett University of South Florida Department of Cell Biology, Microbiology and Molecular Biology 4202 E Fowler Ave BSF 218 Tampa, FL 33620 From yin.alessa from gmail.com Thu Jul 30 14:19:56 2009 From: yin.alessa from gmail.com (yin alessa) Date: Thu Jul 30 15:45:29 2009 Subject: [Zbrafish] Re: sick fish??? References: Message-ID: BTW: the fish were just changed from fry food to adult food. There are about 6 pairs in the tank, definitely not overcrowded. From gclark from cape.com Fri Jul 31 12:02:46 2009 From: gclark from cape.com (Gale Clark) Date: Fri Jul 31 12:11:59 2009 Subject: [Zbrafish] RE: Zfish Digest, Vol 50, Issue 6 : How old are these fish? In-Reply-To: <200907301703.n6UH3Qp17223@net.bio.net> References: <200907301703.n6UH3Qp17223@net.bio.net> Message-ID: <66F1C864F5E54F80A335D9573FC92F4A@D2HZVNB1> Gale Clark gclark@cape.com +1 508 776 1468 -----Original Message----- From: zbrafish-bounces@oat.bio.indiana.edu [mailto:zbrafish-bounces@oat.bio.indiana.edu] On Behalf Of zbrafish-request@oat.bio.indiana.edu Sent: Thursday, July 30, 2009 1:03 PM To: zbrafish@magpie.bio.indiana.edu Subject: Zbrafish Digest, Vol 50, Issue 6 Send Zbrafish mailing list submissions to zbrafish@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/zbrafish or, via email, send a message with subject or body 'help' to zbrafish-request@net.bio.net You can reach the person managing the list at zbrafish-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Zbrafish digest..." Today's Topics: 1. sick fish??? (yin alessa) ---------------------------------------------------------------------- Message: 1 Date: Wed, 29 Jul 2009 19:06:08 -0700 (PDT) From: yin alessa Subject: [Zbrafish] sick fish??? To: bionet-organisms-zebrafish@moderators.isc.org Message-ID: Content-Type: text/plain; charset=ISO-8859-1 Dear All, I had one tank of fish, the males started to get kinky body and become very short, whereas all the females look very healthy. Does anybody know what's going on? Thanks! ------------------------------ _______________________________________________ Zbrafish mailing list Zbrafish@net.bio.net http://www.bio.net/biomail/listinfo/zbrafish End of Zbrafish Digest, Vol 50, Issue 6 *************************************** From gclark from cape.com Fri Jul 31 13:45:32 2009 From: gclark from cape.com (Gale Clark) Date: Fri Jul 31 13:50:12 2009 Subject: [Zbrafish] RE: Z Digest, Vol 50, Issue 7 fish with kinky bodies In-Reply-To: <200907311703.n6VH3jp16214@net.bio.net> References: <200907311703.n6VH3jp16214@net.bio.net> Message-ID: <4F2A8D401E134B91AC14E34BAAFE8EBF@D2HZVNB1> How many days old are they? How many of the (approximately 12) fish are affected? Gale Clark gclark@cape.com +1 508 776 1468 -----Original Message----- From: zbrafish-bounces@oat.bio.indiana.edu [mailto:zbrafish-bounces@oat.bio.indiana.edu] On Behalf Of zbrafish-request@oat.bio.indiana.edu Sent: Friday, July 31, 2009 1:04 PM To: zbrafish@magpie.bio.indiana.edu Subject: Zbrafish Digest, Vol 50, Issue 7 Send Zbrafish mailing list submissions to zbrafish@net.bio.net To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/zbrafish or, via email, send a message with subject or body 'help' to zbrafish-request@net.bio.net You can reach the person managing the list at zbrafish-owner@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Zbrafish digest..." Today's Topics: 1. Luciferase Expression Vector (Kristine Griffett) 2. Re: sick fish??? (yin alessa) ---------------------------------------------------------------------- Message: 1 Date: Thu, 30 Jul 2009 15:34:44 -0400 From: Kristine Griffett Subject: [Zbrafish] Luciferase Expression Vector To: zbrafish@magpie.bio.indiana.edu Message-ID: <95938360-29ED-44CB-B267-1C925EDC5586@mail.usf.edu> Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes Hello All, We would like to use a luciferase expression vector to tag cells in zebrafish embryos and were wondering if anyone has tried to use vectors commercially available from Promega (pRL-CMV) or New England BioLabs (pCMV-gLUC)? Can these vectors be used "as-is" or does the luciferase coding region need to be inserted into a Xenopus or Zebrafish specific vector for expression of the luciferase in the embryo? Kristine Griffett University of South Florida Department of Cell Biology, Microbiology and Molecular Biology 4202 E Fowler Ave BSF 218 Tampa, FL 33620 ------------------------------ Message: 2 Date: Thu, 30 Jul 2009 12:19:56 -0700 (PDT) From: yin alessa Subject: [Zbrafish] Re: sick fish??? To: bionet-organisms-zebrafish@moderators.isc.org Message-ID: Content-Type: text/plain; charset=ISO-8859-1 BTW: the fish were just changed from fry food to adult food. There are about 6 pairs in the tank, definitely not overcrowded. ------------------------------ _______________________________________________ Zbrafish mailing list Zbrafish@net.bio.net http://www.bio.net/biomail/listinfo/zbrafish End of Zbrafish Digest, Vol 50, Issue 7 ***************************************