[Zbrafish] Luciferase Expression Vector

Kristine Griffett via zbrafish%40net.bio.net (by kgriffet from mail.usf.edu)
Thu Jul 30 14:34:44 EST 2009


Hello All,

We would like to use a luciferase expression vector to tag cells in  
zebrafish embryos and were wondering if anyone has tried to use  
vectors commercially available from Promega (pRL-CMV) or New England  
BioLabs (pCMV-gLUC)?  Can these vectors be used "as-is" or does the  
luciferase coding region need to be inserted into a Xenopus or  
Zebrafish specific vector for expression of the luciferase in the  
embryo?

Kristine Griffett
University of South Florida
Department of Cell Biology, Microbiology and Molecular Biology
4202 E Fowler Ave
BSF 218
Tampa, FL 33620



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