[Zbrafish] RE: Z Digest, Vol 50, Issue 7 fish with kinky bodies

Gale Clark via zbrafish%40net.bio.net (by gclark from cape.com)
Fri Jul 31 13:45:32 EST 2009


How many days old are they?  How many of the (approximately 12) fish are
affected?


Gale Clark
gclark from cape.com
+1 508 776 1468

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Sent: Friday, July 31, 2009 1:04 PM
To: zbrafish from magpie.bio.indiana.edu
Subject: Zbrafish Digest, Vol 50, Issue 7

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Today's Topics:

   1. Luciferase Expression Vector (Kristine Griffett)
   2. Re: sick fish??? (yin alessa)


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Message: 1
Date: Thu, 30 Jul 2009 15:34:44 -0400
From: Kristine Griffett <kgriffet from mail.usf.edu>
Subject: [Zbrafish] Luciferase Expression Vector
To: zbrafish from magpie.bio.indiana.edu
Message-ID: <95938360-29ED-44CB-B267-1C925EDC5586 from mail.usf.edu>
Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes

Hello All,

We would like to use a luciferase expression vector to tag cells in  
zebrafish embryos and were wondering if anyone has tried to use  
vectors commercially available from Promega (pRL-CMV) or New England  
BioLabs (pCMV-gLUC)?  Can these vectors be used "as-is" or does the  
luciferase coding region need to be inserted into a Xenopus or  
Zebrafish specific vector for expression of the luciferase in the  
embryo?

Kristine Griffett
University of South Florida
Department of Cell Biology, Microbiology and Molecular Biology
4202 E Fowler Ave
BSF 218
Tampa, FL 33620



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Message: 2
Date: Thu, 30 Jul 2009 12:19:56 -0700 (PDT)
From: yin alessa <yin.alessa from gmail.com>
Subject: [Zbrafish] Re: sick fish???
To: bionet-organisms-zebrafish from moderators.isc.org
Message-ID:
	<ea75d5c3-e763-476c-8361-3d396fad9b98 from p36g2000prn.googlegroups.com>
Content-Type: text/plain; charset=ISO-8859-1

BTW: the fish were just changed from fry food to adult food.  There
are about 6 pairs in the tank, definitely not overcrowded.



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