From a.b.holt from abdn.ac.uk Wed Mar 4 05:31:14 2009 From: a.b.holt from abdn.ac.uk (Holt, Amy Beth) Date: Wed Mar 4 13:06:30 2009 Subject: [Zbrafish] zebrafish pancreas Message-ID: Hi, I need to take tissue from the zebrafish pancreas for an experiment I want to conduct and was wondering is it possible to dissect out the pancreas of the zebrafish by eye? or is it simply too small? If so can it be done under microscope? I am having trouble locating infomation on this. If anyone has any info on this matter or can point me in the right direction of it I would be most grateful! Thanks in advance, Amy The University of Aberdeen is a charity registered in Scotland, No SC013683. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090304/a5f54822/attachment.html From a.b.holt from abdn.ac.uk Fri Mar 6 08:46:59 2009 From: a.b.holt from abdn.ac.uk (Holt, Amy Beth) Date: Fri Mar 6 12:34:26 2009 Subject: [Zbrafish] Thanks! Message-ID: Many thanks to all of you who replied with advice... This has helped alot! Amy Amy Holt Scottish Fish Immunology Research Centre Zoology Building Tillydrone Avenue University of Aberdeen Aberdeen AB24 2TZ Tel office: 01224 273270 Email: a.b.holt@abdn.ac.uk The University of Aberdeen is a charity registered in Scotland, No SC013683. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090306/0213cb11/attachment.html From edwardmellon2 from gmail.com Sat Mar 7 14:43:00 2009 From: edwardmellon2 from gmail.com (Edward Mellon) Date: Mon Mar 9 11:19:20 2009 Subject: [Zbrafish] Draft paper submission is extended (will not be extended further): MULTICONF-09 Message-ID: Draft paper submission is extended (will not be extended further): MULTICONF-09 *Apologies if you receive multiple copies of this email. Please forward to interested people.* The deadline for draft paper submission at the 2009 Multi Conference in Computer Science, Information Technology and Control systems and Computational Science and Computer Engineering (MULTICONF-09) (website: http://www.PromoteResearch.org ) is extended. The conference will be held during July 13-16 2009 in Orlando, FL, USA. We invite draft paper submissions. The event consists of the following conferences: ? International Conference on Artificial Intelligence and Pattern Recognition (AIPR-09) ? International Conference on Automation, Robotics and Control Systems (ARCS-09) ? International Conference on Bioinformatics, Computational Biology, Genomics and Chemoinformatics (BCBGC-09) ? International Conference on Enterprise Information Systems and Web Technologies (EISWT-09) ? International Conference on High Performance Computing, Networking and Communication Systems (HPCNCS-09) ? International Conference on Information Security and Privacy (ISP-09) ? International Conference on Recent Advances in Information Technology and Applications (RAITA-09) ? International Conference on Software Engineering Theory and Practice (SETP-09) ? International Conference on Theory and Applications of Computational Science (TACS-09) ? International Conference on Theoretical and Mathematical Foundations of Computer Science (TMFCS-09) The website http://www.PromoteResearch.org contains more details. Sincerely Edward Mellon Publicity committee -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090307/74365862/attachment.html From rburdine from Princeton.EDU Mon Mar 9 13:48:36 2009 From: rburdine from Princeton.EDU (Burdine, Rebecca D) Date: Mon Mar 9 13:53:29 2009 Subject: [Zbrafish] Here is an odd question for everyone Message-ID: <790DAC09623D0A47ACDB126E3C52D8B60B6488@MBCLUSTER.pu.win.princeton.edu> Hi all, I had a rotation student start in my lab last week. She just informed me that she is deathly allergic to shellfish and carries an Epi-pen at all times for this allergy. Perhaps this should be obvious, but is she in potential danger being around a lab that routinely feeds shrimp to the fish? I don't know enough about shellfish allergies to know if artemia is different allergen-wise, or if it has to be ingested in order to generate anaphylaxis? I'd hate for her to waste her rotation, so I'd love to know what people think. Would she be safe doing a 4-5 year PhD in a zebrafish lab if she has such a severe shellfish allergy? Thanks for the help. Becky --------------------------------------------------- Rebecca D. Burdine, Ph.D. Assistant Professor Dept. of Molecular Biology Princeton University Washington Road Mof 433 Princeton, NJ 08544 Phone: (609) 258-7515 Fax: (609) 258-1343 Email: rburdine@princeton.edu Admin Assistant: Anna Schmedel (609) 258-5028 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090309/605d0626/attachment.html From david from zebrafish.org Mon Mar 9 14:31:06 2009 From: david from zebrafish.org (David Lains) Date: Mon Mar 9 14:35:59 2009 Subject: [Zbrafish] Here is an odd question for everyone In-Reply-To: <790DAC09623D0A47ACDB126E3C52D8B60B6488@MBCLUSTER.pu.win.princeton.edu> References: <790DAC09623D0A47ACDB126E3C52D8B60B6488@MBCLUSTER.pu.win.princeton.edu> Message-ID: <04a601c9a0ed$9ae98ba0$d0bca2e0$@org> Hi Becky I know of several people from aquarium hobby who have shellfish allergies and are allergic to Brine shrimp and cyclop-eeze. Many fish feeds also contain shrimp or krill meal. They still use these products but take appropriate precautions. Hopefully a bit of warning and gloves will be all she needs to participate in Zebrafish research. Best Fishes David Lains <}}}>< Aquaculturist, Research Assistant Zebrafish International Resource Center 5274 University of Oregon Eugene, Or 97403 Email: david@zebrafish.org pH: (541) 346-6028 ext. 18 fax: (541) 346-6151 From: zbrafish-bounces@oat.bio.indiana.edu [mailto:zbrafish-bounces@oat.bio.indiana.edu] On Behalf Of Burdine, Rebecca D Sent: Monday, March 09, 2009 11:49 AM To: zbrafish@magpie.bio.indiana.edu Subject: [Zbrafish] Here is an odd question for everyone Hi all, I had a rotation student start in my lab last week. She just informed me that she is deathly allergic to shellfish and carries an Epi-pen at all times for this allergy. Perhaps this should be obvious, but is she in potential danger being around a lab that routinely feeds shrimp to the fish? I don't know enough about shellfish allergies to know if artemia is different allergen-wise, or if it has to be ingested in order to generate anaphylaxis? I'd hate for her to waste her rotation, so I'd love to know what people think. Would she be safe doing a 4-5 year PhD in a zebrafish lab if she has such a severe shellfish allergy? Thanks for the help. Becky --------------------------------------------------- Rebecca D. Burdine, Ph.D. Assistant Professor Dept. of Molecular Biology Princeton University Washington Road Mof 433 Princeton, NJ 08544 Phone: (609) 258-7515 Fax: (609) 258-1343 Email: rburdine@princeton.edu Admin Assistant: Anna Schmedel (609) 258-5028 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090309/baaadd3d/attachment.html From chuntaoyuan from gmail.com Tue Mar 10 02:44:08 2009 From: chuntaoyuan from gmail.com (Chuntao Yuan) Date: Tue Mar 10 11:28:25 2009 Subject: [Zbrafish] a queation. Thank you. Message-ID: Hi everyone, I met a problem. When I injected a contructed vector with a RFP element. After abou 24 hr when the embryos still developed in the membrane , I fould I could not find any red fluorenscence under the microscope. But after about 3 or 4 days when zebrafish come out from their membrane, I can see strong red fluorencence under microscope. Who can tell me why this happened? Thanks in advance! From yin.alessa from gmail.com Tue Mar 10 23:38:16 2009 From: yin.alessa from gmail.com (yin.alessa@gmail.com) Date: Wed Mar 11 11:28:08 2009 Subject: [Zbrafish] FACS? Message-ID: <57bbb501-42c7-45c1-bb94-e610f353dc55@y38g2000prg.googlegroups.com> Hi All, Does anybody have experience with FACS sorting in zebrafish? I would greatly appreciate any suggestions or protocols for FACS and subsequent RNA extraction. Thank you! Alessa From campjg from email.unc.edu Wed Mar 11 12:38:19 2009 From: campjg from email.unc.edu (Gray Camp) Date: Wed Mar 11 12:47:51 2009 Subject: [Zbrafish] FACS? In-Reply-To: <57bbb501-42c7-45c1-bb94-e610f353dc55@y38g2000prg.googlegroups.com> References: <57bbb501-42c7-45c1-bb94-e610f353dc55@y38g2000prg.googlegroups.com> Message-ID: I am interested in any responses to this as well. Gray On Wed, Mar 11, 2009 at 12:38 AM, wrote: > Hi All, > > Does anybody have experience with FACS sorting in zebrafish? I would > greatly appreciate any suggestions or protocols for FACS and > subsequent RNA extraction. > > Thank you! > > Alessa > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090311/c09280a8/attachment.html From wtpenber from mac.com Wed Mar 11 12:57:13 2009 From: wtpenber from mac.com (W. Todd Penberthy) Date: Wed Mar 11 13:02:56 2009 Subject: [Zbrafish] FACS? In-Reply-To: References: <57bbb501-42c7-45c1-bb94-e610f353dc55@y38g2000prg.googlegroups.com> Message-ID: There is a description for this using 12 hour embryos in Development 124: 4105- (1997) Todd On Mar 11, 2009, at 1:38 PM, Gray Camp wrote: > I am interested in any responses to this as well. > > Gray > > On Wed, Mar 11, 2009 at 12:38 AM, wrote: > Hi All, > > Does anybody have experience with FACS sorting in zebrafish? I would > greatly appreciate any suggestions or protocols for FACS and > subsequent RNA extraction. > > Thank you! > > Alessa > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090311/71540de6/attachment.html From schwartz from lunenfeld.ca Wed Mar 11 13:30:26 2009 From: schwartz from lunenfeld.ca (Cynthia Schwartz) Date: Wed Mar 11 13:33:24 2009 Subject: [Zbrafish] ENU safety precautions Message-ID: Hi. I came across this thread today while looking for safety precautions for working with ENU, did you get any "concrete" information regarding potential exposures and working in a fume hood vs other options. Some of our researchers are interested in working with ENU and mice. Thanks very much, Cynthia Cynthia Schwartz, Ph.D. Laboratory Safety Manager Samuel Lunenfeld Research Institute 60 Murray Street Fifth Floor Room 249 Toronto, Ontario Canada M5T 3L9 Telephone: 416-586-4800 Ext. 8133 schwartz@lunenfeld.ca -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090311/ad4825b5/attachment.html From submit.ajpp from academicjournals.org Wed Mar 18 02:48:24 2009 From: submit.ajpp from academicjournals.org (African Journal of Pharmacy and Pharmacology) Date: Wed Mar 18 11:55:59 2009 Subject: [Zbrafish] Call for Papers Message-ID: *African Journal of Pharmacy and Pharmacology* www.academicjournals.org/AJPP Dear Colleague, *Introducing ‘African Journal of Pharmacy and Pharmacology (AJPP)’* The African Journal of Pharmacy and Pharmacology (AJPP) provide rapid publication of articles in all areas of Pharmaceutical Science. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published approximately one month after acceptance. AJPP is a monthly publication and all articles are peer-reviewed. The following types of papers are considered for publication: · Original articles in basic and applied research. · Critical reviews, surveys, opinions, commentaries and essays. Our objective is to inform authors of the decision on their manuscript(s) within four weeks of submission. Following acceptance, a paper will normally be published in the next issue. Instruction for authors and other details are available on our website www.academicjournals.org/AJPP Prospective authors should send their manuscript(s) to ajpp@academicjournals.org , ajpp.academicjournals@gmail.com *Open Access* One key request of researchers across the world is unrestricted access to research publications. AJPP is fully committed Open Access Initiative by providing free access to all articles (both abstract and full PDF text) as soon as they are published. We ask you to support this initiative by publishing your papers in this journal. *Publication Alert* We will be glad to send you a publication alert showing the table of content with link to the various abstracts and full PDF text of articles published in each issue. Kindly send us an email if you will like to receive publication alert. Best regards, Dr. Seetulsingh- Goorah Editor, African Journal of Pharmacy and Pharmacology http://www.academicjournals.org/AJPP E-mail: ajpp@academicjournals.org, ajpp.academicjournals@gmail.com, ajpp_acadjourn@yahoo.com *http://www.academicjournals.org/AJPP * * * * * * * * * -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090318/798a19ac/attachment.html From rburdine from Princeton.EDU Mon Mar 23 10:19:46 2009 From: rburdine from Princeton.EDU (Burdine, Rebecca D) Date: Mon Mar 23 10:38:13 2009 Subject: [Zbrafish] Antibody production Message-ID: <790DAC09623D0A47ACDB126E3C52D8B614CAED@MBCLUSTER.pu.win.princeton.edu> Hi everyone, Does anyone have a company they would recommend for making monoclonal antibodies? I am hoping to find one that is reasonably priced. Thanks, Becky --------------------------------------------------- Rebecca D. Burdine, Ph.D. Assistant Professor Dept. of Molecular Biology Princeton University Washington Road Mof 433 Princeton, NJ 08544 Phone: (609) 258-7515 Fax: (609) 258-6730 Email: rburdine@princeton.edu Admin Assistant: Anna Schmedel (609) 258-5028 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090323/382bb8d2/attachment.html From pow.joshi from gmail.com Tue Mar 24 14:12:35 2009 From: pow.joshi from gmail.com (pow) Date: Tue Mar 24 14:16:57 2009 Subject: [Zbrafish] Re: a queation. Thank you. References: Message-ID: <8b42a1e1-6141-4bf0-ae5b-0b44ad9882d7@c9g2000yqm.googlegroups.com> On Mar 10, 3:44 am, Chuntao Yuan wrote: > Hi everyone, > > I met a problem. When I injected a contructed vector with a RFP > element. After abou 24 hr when the embryos still developed in the > membrane , I fould I could not find any red fluorenscence under the > microscope. But after about 3 or 4 days when zebrafish come out from > their membrane, I can see strong red fluorencence under microscope. > > Who can tell me why this happened? > > Thanks in advance! Hi, well, wondering if you use generic/ubiquitous promoters like CMV or specific. Give us more info Pow From gottalovelife from gmail.com Wed Mar 25 11:40:01 2009 From: gottalovelife from gmail.com (Kim) Date: Wed Mar 25 11:53:24 2009 Subject: [Zbrafish] Oxidative stress kits Message-ID: <7df549e2-ece7-4592-8636-be474fd65bbe@c18g2000prh.googlegroups.com> Hello all; I am looking for a reliable oxidative stress kit to buy to test zebrafish embryos. Does anyone have any suggestions? Thank you! From mwinandy from ulg.ac.be Wed Mar 25 08:27:10 2009 From: mwinandy from ulg.ac.be (Marie Winandy) Date: Wed Mar 25 11:53:46 2009 Subject: [Zbrafish] Mass Embryo Production System In-Reply-To: <200902241703.n1OH3ue27701@net.bio.net> References: <200902241703.n1OH3ue27701@net.bio.net> Message-ID: <49CA312E.2090204@ulg.ac.be> An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090325/4c75ee6a/attachment.html -------------- next part -------------- A non-text attachment was scrubbed... Name: mwinandy.vcf Type: text/x-vcard Size: 398 bytes Desc: not available Url : http://www.bio.net/bionet/mm/zbrafish/attachments/20090325/4c75ee6a/mwinandy.bin From jedgold from gmail.com Thu Mar 26 10:09:55 2009 From: jedgold from gmail.com (Jed G) Date: Thu Mar 26 11:59:33 2009 Subject: [Zbrafish] Re: Preparation of zebrafish embryos for oxidative stress assays References: Message-ID: <077fc8a9-0330-4bbd-bbbb-e0527235b84a@r34g2000vba.googlegroups.com> On Feb 23, 2:01?pm, Kim wrote: > Hello all; > > ?Does anyone have experience preparing extract from zebrafish embryos > for oxidative stress assays? What sort of assays? From jedgold from gmail.com Thu Mar 26 10:13:34 2009 From: jedgold from gmail.com (Jed G) Date: Thu Mar 26 11:59:57 2009 Subject: [Zbrafish] Re: Antibody production References: Message-ID: On Mar 23, 11:19?am, "Burdine, Rebecca D" wrote: > Hi everyone, > > Does anyone have a company they would recommend for making monoclonal > antibodies? ? I am hoping to find one that is reasonably priced. > > Thanks, > > Becky > > --------------------------------------------------- > Rebecca D. Burdine, Ph.D. > Assistant Professor > Dept. of Molecular Biology > Princeton University > Washington Road Mof 433 > Princeton, NJ 08544 > Phone: (609) 258-7515 > Fax: (609) 258-6730 > Email: rburd...@princeton.edu > Admin Assistant: Anna Schmedel (609) 258-5028 We've used 21st Century Biochemicals, but have had only medium success (1 very good one of 5 zfish proteins, one ok, the rest we're redoing). The lab next door had 4 of 5 successes. 21st C. has been quite responsive, we've just not had good luck. Jed Goldstone Woods Hole Oceanographic Institution From jedgold from gmail.com Thu Mar 26 13:12:50 2009 From: jedgold from gmail.com (Jed G) Date: Thu Mar 26 13:14:24 2009 Subject: [Zbrafish] Re: Antibody production References: Message-ID: <5477320d-98e8-482c-91d7-cdef20e11477@n20g2000vba.googlegroups.com> Sorry, I made an error in reading your question. All of our antibodies are polyclonal, not monoclonal. Jed From zhangch from ohsu.edu Fri Mar 27 16:31:57 2009 From: zhangch from ohsu.edu (Chao Zhang) Date: Fri Mar 27 16:51:30 2009 Subject: [Zbrafish] Morpholino dose--ng or pg per embryo Message-ID: Hi: I have a Morpholino oligo at 8300g/mol. Working solution is 0.5mM, 1nL is injected into each embryo. That should be 0.5mM * 1nL * 8300g/mol=4.15* 10E-12 g/embryo(4.15pg/embryo), right? Why zebrafish guys always say 4* 10E-9 g/embryo(4ng/embryo)? Anybody could give me an answer? Thanks. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090327/206c8e85/attachment.html From challa.anilkumar from gmail.com Mon Mar 30 03:18:00 2009 From: challa.anilkumar from gmail.com (AKC) Date: Mon Mar 30 08:53:49 2009 Subject: [Zbrafish] Mouth deformation Message-ID: <8b4d6af5-0a6a-4d10-85fe-de6ffed80c50@y34g2000prb.googlegroups.com> Hello! In the last couple of days I observed that a fish in one of my tanks have developed a strange mouth deformation. The first thing I noticed was that the fish has its mouth open all the time. When I observed closely it seemed like the lower jaw bones got dislodged. I couldn't take good pictures, so I took this short video: http://sites.google.com/site/hydzebrafish/Home/L1000299.MOV The fish with the deformity is nearer to the camera when the movie begins. I tried to maintain focus on the same fish. Does anyone know what the problem with this fish is? Thanks in advance. Anil From chi-bin.chien from neuro.utah.edu Fri Mar 27 17:58:32 2009 From: chi-bin.chien from neuro.utah.edu (Chi-Bin Chien) Date: Mon Mar 30 08:54:12 2009 Subject: [Zbrafish] Morpholino dose--ng or pg per embryo In-Reply-To: References: Message-ID: 0.5 mM = 0.5 millimolar (*not* micromolar) = 0.5 x 10E-3 mol/l Your calculation was low by a factor of 1000. Chi-Bin Chien On Mar 27, 2009, at 3:31 PM, Chao Zhang wrote: > Hi: > I have a Morpholino oligo at 8300g/mol. > Working solution is 0.5mM, 1nL is injected into each embryo. > That should be 0.5mM * 1nL * 8300g/mol=4.15* 10E-12 g/embryo(4.15pg/ > embryo), right? > Why zebrafish guys always say 4* 10E-9 g/embryo(4ng/embryo)? > Anybody could give me an answer? > Thanks. > > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090327/6da5be99/attachment.html From jason.cockington from gmail.com Sun Mar 29 19:02:56 2009 From: jason.cockington from gmail.com (Leviathan) Date: Mon Mar 30 08:54:37 2009 Subject: [Zbrafish] Re: Mass Embryo Production System References: <200902241703.n1OH3ue27701@net.bio.net> Message-ID: Hi All, I'd be keen to here your views on the MEPS or Mini MEPS also. We currently produce embryos via tank spawning (60 fish/Tank) over a trap. This occurs daily across several different time points. We find although fairly reliable, we still get hit and miss days. So, those of you that are currently using a MEPS: Do you reliably get lays everyday? Roughly how many eggs do you get in a lay? How much control do you get with regards to the age of the embryos when harvested? ie are they mixed, or are you able to collect in a tight enough window to get 1cell embryos? thanks, jason From lasse from scienceshow.dk Sat Mar 28 09:23:56 2009 From: lasse from scienceshow.dk (Lasse) Date: Mon Mar 30 08:55:02 2009 Subject: [Zbrafish] Re: Morpholino dose--ng or pg per embryo References: Message-ID: On Mar 27, 10:31?pm, "Chao Zhang" wrote: > Hi: > I have a Morpholino oligo at 8300g/mol. > Working solution is 0.5mM, 1nL is injected into each embryo. > That should be 0.5mM * 1nL * 8300g/mol=4.15* 10E-12 g/embryo(4.15pg/embryo), right? > Why zebrafish guys always say 4* 10E-9 g/embryo(4ng/embryo)? > Anybody could give me an answer? > Thanks. Dear Chao Zhang I think there might be an error in your calculation... At least according to the following way of viewing the problem, ng would be the correct unit: 0.5mM = 5*E-4 M 1nL = 1*E-9 L 8300 g/mole = 8.3*E+3 g/mole thus 5 * E-4 * 1 * E-9 * 8.3 * E+3 = 5 * 1 * 8.3 * E-4 * E-9 * E+3 = 41,5 * E-10 = 4,15 ng Hope this makes sense :). All my best, and good luck with your morpholino experiments! From lhqkey from gmail.com Sat Mar 28 14:33:29 2009 From: lhqkey from gmail.com (Haiqiong) Date: Mon Mar 30 08:55:18 2009 Subject: [Zbrafish] Re: Morpholino dose--ng or pg per embryo References: Message-ID: <6ca0acbc-8723-4f9b-9fbc-3e14009ffdec@p20g2000yqi.googlegroups.com> Hi, It should be 4.15 ng/embryo. 0.5 * 10E-3 mol/ L * 10E-9 L * 8300 g/mol = 4.15 *10E-9 g = 4.15 ng On Mar 27, 5:31?pm, "Chao Zhang" wrote: > Hi: > I have a Morpholino oligo at 8300g/mol. > Working solution is 0.5mM, 1nL is injected into each embryo. > That should be 0.5mM * 1nL * 8300g/mol=4.15* 10E-12 g/embryo(4.15pg/embryo), right? > Why zebrafish guys always say 4* 10E-9 g/embryo(4ng/embryo)? > Anybody could give me an answer? > Thanks. From lihu from umbi.umd.edu Sun Mar 29 10:20:10 2009 From: lihu from umbi.umd.edu (lihu@umbi.umd.edu) Date: Mon Mar 30 08:55:36 2009 Subject: [Zbrafish] Re: Zbrafish Digest, Vol 46, Issue 12 In-Reply-To: <200903281703.n2SH3Jh10895@net.bio.net> References: <200903281703.n2SH3Jh10895@net.bio.net> Message-ID: Re: Morpholino dose--ng or pg per embryo (Chao Zhang) Hi Zhang Chao, How are you? You did wrong calculation, 0.5mM * 1nL * 8300g/mol should be 4.15 ng. Please recalculate. Best Huiqing ----- Original Message ----- From: zbrafish-request@oat.bio.indiana.edu Date: Saturday, March 28, 2009 1:03 pm Subject: Zbrafish Digest, Vol 46, Issue 12 To: zbrafish@magpie.bio.indiana.edu > Send Zbrafish mailing list submissions to > zbrafish@net.bio.net > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/zbrafish > or, via email, send a message with subject or body 'help' to > zbrafish-request@net.bio.net > > You can reach the person managing the list at > zbrafish-owner@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Zbrafish digest..." > > > Today's Topics: > > 1. Morpholino dose--ng or pg per embryo (Chao Zhang) > > > ----------------------------------------------------------------- > ----- > > Message: 1 > Date: Fri, 27 Mar 2009 14:31:57 -0700 > From: "Chao Zhang" > Subject: [Zbrafish] Morpholino dose--ng or pg per embryo > To: > Message-ID: BE06.ohsu.edu>Content-Type: text/plain; charset="gb2312" > > Hi: > I have a Morpholino oligo at 8300g/mol. > Working solution is 0.5mM, 1nL is injected into each embryo. > That should be 0.5mM * 1nL * 8300g/mol=4.15* 10E-12 > g/embryo(4.15pg/embryo), right? > Why zebrafish guys always say 4* 10E-9 g/embryo(4ng/embryo)? > Anybody could give me an answer? > Thanks. > -------------- next part -------------- > An HTML attachment was scrubbed... > URL: > http://www.bio.net/bionet/mm/zbrafish/attachments/20090327/206c8e85/attachment-0001.html > > ------------------------------ > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > > End of Zbrafish Digest, Vol 46, Issue 12 > **************************************** > Huiqing Li Ph.D Candidate Center of Marine Biotechnology 701 E Pratt St. Baltimore, MD 21202 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/zbrafish/attachments/20090329/b0434afe/attachment.html From Christian.Lawrence from childrens.harvard.edu Mon Mar 30 09:26:58 2009 From: Christian.Lawrence from childrens.harvard.edu (Lawrence, Christian) Date: Mon Mar 30 11:44:57 2009 Subject: [Zbrafish] Mouth deformation In-Reply-To: <8b4d6af5-0a6a-4d10-85fe-de6ffed80c50@y34g2000prb.googlegroups.com> Message-ID: Yes, we see it from time to time. I believe that it is indeed the lower jaw getting dislodged initially. I think it is likely that there is some underlying genetic factor involved because when we do see these fish - and it isn't often - there will usually be a few fish in the same tank that develop this over time. Christian Lawrence Aquatic Resources Program Children's Hospital Boston 320 Longwood Avenue Boston, MA 02115 617.919.2738 (office) 617.730.0836 (fax) christian.lawrence@childrens.harvard.edu On 3/30/09 4:18 AM, "AKC" wrote: Hello! In the last couple of days I observed that a fish in one of my tanks have developed a strange mouth deformation. The first thing I noticed was that the fish has its mouth open all the time. When I observed closely it seemed like the lower jaw bones got dislodged. I couldn't take good pictures, so I took this short video: http://sites.google.com/site/hydzebrafish/Home/L1000299.MOV The fish with the deformity is nearer to the camera when the movie begins. I tried to maintain focus on the same fish. Does anyone know what the problem with this fish is? Thanks in advance. Anil _______________________________________________ Zbrafish mailing list Zbrafish@net.bio.net http://www.bio.net/biomail/listinfo/zbrafish The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. From rburdine from Princeton.EDU Mon Mar 30 09:36:23 2009 From: rburdine from Princeton.EDU (Burdine, Rebecca D) Date: Mon Mar 30 11:45:21 2009 Subject: [Zbrafish] Re: Mass Embryo Production System In-Reply-To: References: <200902241703.n1OH3ue27701@net.bio.net> Message-ID: <790DAC09623D0A47ACDB126E3C52D8B614CD8F@MBCLUSTER.pu.win.princeton.edu> We aren't using the MEPS system, but we are using inserts from AHAB for the 2L tank to spawn wildtypes every day. What we find is like almost any other mating: 1. The fish have to get used to the setup 2. Whether or not you get eggs reliably depends on the fish strain, the fish age, and how well the fish are fed. 3. We usually set up for the next morning around 4-5pm. (Our lights come on at 10am during spring forward) We do get a few that lay during the night, but most seem to get on schedule fairly rapidly. One trick that works really well for us is the following: We remove the fish tanks from the system and place the fish into a breeding insert into a 2L tank that contains 1/2 system water and 1/2 straight RO1 water. We leave them off the system for breeding. Keeping the water on the shallow side in the breeding insert with several plastic plants encourages egg lay and helps prevent fish fights. Probably preaching to the converted, but there you go. Becky --------------------------------------------------- Rebecca D. Burdine, Ph.D. Assistant Professor Dept. of Molecular Biology Princeton University Washington Road Mof 433 Princeton, NJ 08544? ? Phone:?(609) 258-7515 Fax: (609) 258-6730 Email: rburdine@princeton.edu Admin Assistant: Anna Schmedel (609) 258-5028 > -----Original Message----- > From: zbrafish-bounces@oat.bio.indiana.edu [mailto:zbrafish- > bounces@oat.bio.indiana.edu] On Behalf Of Leviathan > Sent: Sunday, March 29, 2009 8:03 PM > To: bionet-organisms-zebrafish@moderators.isc.org > Subject: [Zbrafish] Re: Mass Embryo Production System > > Hi All, > > I'd be keen to here your views on the MEPS or Mini MEPS also. > > We currently produce embryos via tank spawning (60 fish/Tank) over a > trap. This occurs daily across several different time points. > > We find although fairly reliable, we still get hit and miss days. > > So, those of you that are currently using a MEPS: > > Do you reliably get lays everyday? > > Roughly how many eggs do you get in a lay? > > How much control do you get with regards to the age of the embryos > when harvested? ie are they mixed, or are you able to collect in a > tight enough window to get 1cell embryos? > > thanks, > > jason > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish From anne_streit from web.de Tue Mar 31 02:54:08 2009 From: anne_streit from web.de (Anne Streit) Date: Tue Mar 31 11:06:13 2009 Subject: [Zbrafish] Mouth deformation Message-ID: <1325107399@web.de> Hmm, I unfortunately could not open the movie, but from the description I assume it could also be one symptome of fish-TBC. I have seen z-fish with TBC that show open jaws. (Hope for you that it?s not!!!). But then I think you would also have death fish from time to time and other malformations, ulcera and so on.. good luck! Anne > -----Urspr?ngliche Nachricht----- > Von: "Lawrence, Christian" > Gesendet: 30.03.09 18:49:20 > An: "bionet-organisms-zebrafish@moderators.isc.org" > Betreff: Re: [Zbrafish] Mouth deformation > Yes, we see it from time to time. I believe that it is indeed the lower jaw getting dislodged initially. I think it is likely that there is some underlying genetic factor involved because when we do see these fish - and it isn't often - there will usually be a few fish in the same tank that develop this over time. > > > Christian Lawrence > Aquatic Resources Program > Children's Hospital Boston > 320 Longwood Avenue > Boston, MA 02115 > 617.919.2738 (office) > 617.730.0836 (fax) > christian.lawrence@childrens.harvard.edu > > > > > > > On 3/30/09 4:18 AM, "AKC" wrote: > > > > > > Hello! > > In the last couple of days I observed that a fish in one of my tanks > have developed a strange mouth deformation. The first thing I noticed > was that the fish has its mouth open all the time. When I observed > closely it seemed like the lower jaw bones got dislodged. > > I couldn't take good pictures, so I took this short video: > http://sites.google.com/site/hydzebrafish/Home/L1000299.MOV > > The fish with the deformity is nearer to the camera when the movie > begins. I tried to maintain focus on the same fish. > > Does anyone know what the problem with this fish is? > > Thanks in advance. > Anil > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > > > The information in this e-mail is intended only for the person to whom it is > addressed. If you believe this e-mail was sent to you in error and the e-mail > contains patient information, please contact the Partners Compliance HelpLine at > http://www.partners.org/complianceline . If the e-mail was sent to you in error > but does not contain patient information, please contact the sender and properly > dispose of the e-mail. > > > > > > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish > __________________________________________________________________________ Verschicken Sie SMS direkt vom Postfach aus - in alle deutschen und viele ausl?ndische Netze zum gleichen Preis! https://produkte.web.de/webde_sms/sms From anne_streit from web.de Tue Mar 31 03:06:18 2009 From: anne_streit from web.de (Anne Streit) Date: Tue Mar 31 11:06:35 2009 Subject: [Zbrafish] FW: injection volume calibration Message-ID: <1325134427@web.de> > -----Urspr?ngliche Nachricht----- > Von: "Anne Streit" > Gesendet: 31.03.09 10:02:31 > An: Zbrafish@net.bio.net > Betreff: injection volume calibration > > Hi everybody! > > I also have a question concerning morpholino injection: > I calibrate my injection system (femtojet by eppendorf) with the help of > a scalebar injecting a drop in halocarbon oil... > but I found different equations for the calculation of the drob volume by measuring the > drob radius/diameter .. > does anyone have a reasonable calibration curve? That would be great! > > best regards Anne __________________________________________________________________________ Verschicken Sie SMS direkt vom Postfach aus - in alle deutschen und viele ausl?ndische Netze zum gleichen Preis! https://produkte.web.de/webde_sms/sms From tgenade from gmail.com Tue Mar 31 08:28:31 2009 From: tgenade from gmail.com (Tyrone) Date: Tue Mar 31 11:07:31 2009 Subject: [Zbrafish] antibody cell markers Message-ID: Hello, Can anyone suggest antibody markers for keratinocytes and melanophores that work in Zebrafish? Anyone tried S100B for melanophores? I know it labels astrocytes very nicely in my fish (Nothobranchius guentheri). What about anti-keratin 3 for keratinocytes? Many thanks