[Zbrafish] Re: a queation. Thank you.

pow via zbrafish%40net.bio.net (by pow.joshi from gmail.com)
Tue Mar 24 14:12:35 EST 2009


On Mar 10, 3:44 am, Chuntao Yuan <chuntaoy... from gmail.com> wrote:
> Hi everyone,
>
> I met a problem. When I injected a contructed vector with a RFP
> element. After abou 24 hr when the embryos still developed in the
> membrane , I fould I could not find any red fluorenscence under the
> microscope.  But after about 3 or 4 days when zebrafish come out from
> their membrane, I can see strong red fluorencence  under microscope.
>
> Who can tell me why this happened?
>
> Thanks in advance!

Hi, well, wondering if you use generic/ubiquitous promoters like CMV
or specific. Give us more info

Pow



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