[Zbrafish] Morpholino dose--ng or pg per embryo

Chi-Bin Chien via zbrafish%40net.bio.net (by chi-bin.chien from neuro.utah.edu)
Fri Mar 27 17:58:32 EST 2009


0.5 mM = 0.5 millimolar (*not* micromolar) = 0.5 x 10E-3 mol/l

Your calculation was low by a factor of 1000.

Chi-Bin Chien

On Mar 27, 2009, at 3:31 PM, Chao Zhang wrote:
> Hi:
> I have a Morpholino oligo at 8300g/mol.
> Working solution is 0.5mM, 1nL is injected into each embryo.
> That should be 0.5mM * 1nL * 8300g/mol=4.15* 10E-12 g/embryo(4.15pg/ 
> embryo), right?
> Why zebrafish guys always say 4* 10E-9 g/embryo(4ng/embryo)?
> Anybody could give me an answer?
> Thanks.
>
> <ATT00001.txt>

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