[Zbrafish] Re: Morpholino dose--ng or pg per embryo
(by lasse from scienceshow.dk)
Sat Mar 28 09:23:56 EST 2009
On Mar 27, 10:31 pm, "Chao Zhang" <zhan... from ohsu.edu> wrote:
> I have a Morpholino oligo at 8300g/mol.
> Working solution is 0.5mM, 1nL is injected into each embryo.
> That should be 0.5mM * 1nL * 8300g/mol=4.15* 10E-12 g/embryo(4.15pg/embryo), right?
> Why zebrafish guys always say 4* 10E-9 g/embryo(4ng/embryo)?
> Anybody could give me an answer?
Dear Chao Zhang
I think there might be an error in your calculation... At least
according to the following way of viewing the problem, ng would be the
0.5mM = 5*E-4 M
1nL = 1*E-9 L
8300 g/mole = 8.3*E+3 g/mole thus
5 * E-4 * 1 * E-9 * 8.3 * E+3 = 5 * 1 * 8.3 * E-4 * E-9 * E+3 = 41,5 *
E-10 = 4,15 ng
Hope this makes sense :).
All my best, and good luck with your morpholino experiments!
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