[Zbrafish] Re: Morpholino dose--ng or pg per embryo

Lasse via zbrafish%40net.bio.net (by lasse from scienceshow.dk)
Sat Mar 28 09:23:56 EST 2009


On Mar 27, 10:31 pm, "Chao Zhang" <zhan... from ohsu.edu> wrote:
> Hi:
> I have a Morpholino oligo at 8300g/mol.
> Working solution is 0.5mM, 1nL is injected into each embryo.
> That should be 0.5mM * 1nL * 8300g/mol=4.15* 10E-12 g/embryo(4.15pg/embryo), right?
> Why zebrafish guys always say 4* 10E-9 g/embryo(4ng/embryo)?
> Anybody could give me an answer?
> Thanks.

Dear Chao Zhang

I think there might be an error in your calculation... At least
according to the following way of viewing the problem, ng would be the
correct unit:

0.5mM        = 5*E-4 M
1nL             = 1*E-9 L
8300 g/mole = 8.3*E+3 g/mole       thus

5 * E-4 * 1 * E-9 * 8.3 * E+3 = 5 * 1 * 8.3 * E-4 * E-9 * E+3 = 41,5 *
E-10 = 4,15 ng

Hope this makes sense :).

All my best, and good luck with your morpholino experiments!



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