[Zbrafish] Re: Morpholino dose--ng or pg per embryo

Haiqiong via zbrafish%40net.bio.net (by lhqkey from gmail.com)
Sat Mar 28 14:33:29 EST 2009


Hi,

It should be  4.15 ng/embryo.

0.5 * 10E-3 mol/ L * 10E-9 L * 8300 g/mol = 4.15 *10E-9 g = 4.15 ng


On Mar 27, 5:31 pm, "Chao Zhang" <zhan... from ohsu.edu> wrote:
> Hi:
> I have a Morpholino oligo at 8300g/mol.
> Working solution is 0.5mM, 1nL is injected into each embryo.
> That should be 0.5mM * 1nL * 8300g/mol=4.15* 10E-12 g/embryo(4.15pg/embryo), right?
> Why zebrafish guys always say 4* 10E-9 g/embryo(4ng/embryo)?
> Anybody could give me an answer?
> Thanks.




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