[Zbrafish] Re: Zbrafish Digest, Vol 46, Issue 12

lihu from umbi.umd.edu via zbrafish%40net.bio.net (by lihu from umbi.umd.edu)
Sun Mar 29 10:20:10 EST 2009


Re: Morpholino dose--ng or pg per embryo (Chao Zhang)

Hi Zhang Chao,
How are you? You did wrong calculation, 0.5mM * 1nL * 8300g/mol should be 4.15 ng. Please recalculate. 
Best
Huiqing


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From: zbrafish-request from oat.bio.indiana.edu
Date: Saturday, March 28, 2009 1:03 pm
Subject: Zbrafish Digest, Vol 46, Issue 12
To: zbrafish from magpie.bio.indiana.edu

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>    1. Morpholino dose--ng or pg per embryo (Chao Zhang)
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> Message: 1
> Date: Fri, 27 Mar 2009 14:31:57 -0700
> From: "Chao Zhang" <zhangch from ohsu.edu>
> Subject: [Zbrafish] Morpholino dose--ng or pg per embryo
> To: <zbrafish from magpie.bio.indiana.edu>
> Message-ID: <F58AB4839D379649A9E190A61925764A02044E from EX-
> BE06.ohsu.edu>Content-Type: text/plain; charset="gb2312"
> 
> Hi:
> I have a Morpholino oligo at 8300g/mol.
> Working solution is 0.5mM, 1nL is injected into each embryo.
> That should be 0.5mM * 1nL * 8300g/mol=4.15* 10E-12 
> g/embryo(4.15pg/embryo), right?
> Why zebrafish guys always say 4* 10E-9 g/embryo(4ng/embryo)?
> Anybody could give me an answer? 
> Thanks.
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Huiqing Li
Ph.D Candidate
Center of Marine Biotechnology
701 E Pratt St. 
Baltimore, MD 21202
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