[Zbrafish] Re Imaging Zebrafish

James Lister via zbrafish%40net.bio.net (by jalister from vcu.edu)
Wed May 27 12:17:08 EST 2009


Here is a link to the sideview plate that Christian and Caroline
mentioned...

http://www.sideviewmicroplate.com/

-jim 

> -----Original Message-----
> From: zbrafish-bounces from oat.bio.indiana.edu 
> [mailto:zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of C Parkin
> Sent: Wednesday, May 27, 2009 4:12 AM
> To: zbrafish from oat.bio.indiana.edu
> Subject: [Zbrafish] Re Imaging Zebrafish
> 
> Hi Sarah,
> 
> I also think the best way to image live fish is to put them 
> in agarose - but
> remember to use low melting point agarose - or you'll fry 
> your fish! I tend to
> melt a batch and put it in microtubes and keep it at 50C 
> ready for use (either
> hotblock or waterbath).  Don't add tricaine till you need it! 
> I find mounting
> fish when the agarose is ready is very quick.
> Then it then depends if you have an upright or inverted 
> scope.  For inverted I
> like to use glass bottom mini petri dishes. For upright you 
> can do the same and
> turn it upside down or use a water immersion lens.  
> If you want to image lots of embryos on their side you could 
> try a sideview
> plate (google it) or a optically clear 96 well plate and spin 
> them down at low
> speed on a centrifuge - the majority should be laying on 
> their side (all
> dependant on age though - works best for 2-4 days old 
> embryos, or older if the
> swim bladder isn't inflated). This is only really suitable 
> for an inverted
> scope.
> It is possible to use a silicone spacer (to make a small 
> well) and put the
> embryo in the well (in E3) and put a cover slip on top. But 
> your fish might
> move more and can't be left for a long time like this.
> 
> I hope that's helpful,
> Caroline
> 
> 
> > 
> > 
> > On May 22, 8:27 am, FritzXC23 <frits... from gettysburg.edu> wrote:
> > > Hi All!
> > >
> > > My name is Sarah Fritz and I am undergraduate student 
> from Gettysburg
> > > College. I am currently working on a research project 
> that involves
> > > imaging fluorescent zebrafish hair cells. I am wondering 
> if anyone has
> > > suggestions about the best way to get 5 dpf fish to lay 
> on their sides
> > > without having to mount them. Thanks!
> > >
> > > Sarah Fritz
> > 
> > 
> > 
> > 
> > ------------------------------
> > 
> > Message: 2
> > Date: Sat, 23 May 2009 12:20:43 -0700
> > From: "Chao Zhang" <zhangch from ohsu.edu>
> > Subject: [Zbrafish] zebrafish inbred line
> > To: <zbrafish from oat.bio.indiana.edu>
> > Message-ID: 
> <F58AB4839D379649A9E190A61925764A020459 from EX-BE06.ohsu.edu>
> > Content-Type: text/plain; charset="gb2312"
> > 
> > Is zebrafish inbred line available for our research.
> > AB, Tab or TU?
> > Thanks.
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