[Zbrafish] On raising Zebrafish larvae

Burdine, Rebecca D via zbrafish%40net.bio.net (by rburdine from Princeton.EDU)
Wed Sep 9 10:52:30 EST 2009

I would like to add that we raise our larvae on dry food until day 20 or so.  We do not use paramecium.  Around day 20 we start feeding brine shrimp in addition to the dry food..

We have had good luck with AZ100 and BioKyowa for fish 5-10 days old.  We have used various sized Zeigler AP100 dry food for 10-30 days.

We typically get survival over 80% depending on the strain.


Rebecca D. Burdine, Ph.D.
Assistant Professor
Dept. of Molecular Biology
Princeton University
Washington Road Mof 433
Princeton, NJ 08544 
Phone: (609) 258-7515
Fax: (609) 258-6730
Email: rburdine from princeton.edu
Admin Assistant: Anna Schmedel (609) 258-5028

> -----Original Message-----
> From: zbrafish-bounces from oat.bio.indiana.edu [mailto:zbrafish-
> bounces from oat.bio.indiana.edu] On Behalf Of Lawrence, Christian
> Sent: Tuesday, September 08, 2009 10:51 PM
> To: chubbyjayu; bionet-organisms-zebrafish from moderators.isc.org
> Subject: Re: [Zbrafish] On raising Zebrafish larvae
> In all likelihood, the fish are basically starving.
> At 28C, they start exogenous feeding at 5 days, but also continue to derive
> nutritional benefit from the yolk sac, but this is generally gone by day 7.  If you
> don't give them any food at all, they will starve at day 10 or so.
> No matter how dense, standard, bacteria-fed Paramecium does not meet the
> considerable nutritional demands of the fish beyond the first few days of feeding.
> If you do not present the larvae with something else with a better nutritional
> profile (higher protein, and specific lipid content) at this point, they will starve.  If
> all of your fish die by 12-14 days, then that is likely what is happening.  The
> Paramecium typically gets them out a day or two longer than the 10 day mark.
> Even if you manage to keep them alive beyond that, they won't grow until you
> give them something with an adequate nutritional profile.  You're just prolonging
> the starvation.
> If you start feeding Paramecium at day 5 (as soon as they are swimming), you
> should be presenting them with another item, in small, frequent applications,
> whether it be first stage Artemia nauplii or a processed larval feed, by 7-9 days.
> Once you begin do this, you should put them on flow at a slow drip to maintain
> stable (but not necessarily pristine) water quality.   Once it becomes apparent that
> they are feeding on the new items (full guts, rapid growth), you can stop the
> paramecium applications, increase the amounts of the new feed per feeding,
> and slowly increase flow rates as the fish grow.
> The weaning from a low nutritional quality first feed like Paramecium to
> something better is the key to success in larval rearing.  If you don't do it correctly
> or well, your survival rates will always be poor.
> Chris
> On 9/7/09 12:11 AM, "chubbyjayu" <chubbyjayu from gmail.com> wrote:
> Hello all!
> I have been trying to raise zebrafish larvae in the lab
> unsuccessfully. The problem is that the larvae tend to die 12 - 14
> days post fertilization and there seems to be no logical cause for the
> death.
> I check the paramecium stocks for coleps everyday, but see none. We
> have even tried raising the zebrafish  larvae in embryo medium (made
> as per the zebrafish book), but even then we see that the larvae die
> out within 14 days.
> Could you please help me figure out where I am going wrong?
> Jayasree
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