[Zbrafish] protocol needed for phospho-smad staining

Chunyue via zbrafish%40net.bio.net (by yin.alessa from gmail.com)
Tue Sep 29 19:10:46 EST 2009


Hi All,

I tried to do immunohistochemistry using phospho-smad antibody
(purchased from Cell Signal Technology).  After several trials, I
couldn't get any staining.  Is anybody willing to share their
protocols?

BTW: I prefer to do the staining on whole-mount or vibrotome sections.

Thanks a lot!



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