[Zbrafish] Zebrafish females not looking gravid

[Bill Trevarrow]

Hi Val,

You might want to check that you lights are going off at night.

Many automatic light systems have overrides that can accidentally be  
set to keep the lights on all night. This will prevent the fish from  
breeding and will not be obvious since normally no one is there when  
the lights are supposed to be off.

On Apr 5, 2010, at 10:23 PM, val wrote:

> hi
> The adult zebrafish females in our facility do not seem to be getting
> 'chubby'.It is a problem indentifying the females to set them up for
> breeding. We have been feeding them about 3-4 times a day with variety
> of diets including Artemia,micropellets,Daphnia and tubifex worms.
>
> Moreover the fish have stopped breeding and there has been no egg
> production for the last 6 months. They show no sign of infection or
> disease. It is hard to tell if there is any chasing in the breeding
> tanks at all. The male:female ratio in the breeding tanks is 2:1. We
> have also setup mass breeding (4females+ 8males) in the tanks but to
> no success.
> We have been treating them with salt water once every week for the
> past 3 weeks to rule out any chance of infection. We tested the water
> for all relevant parameters and the water condition seems optimal.
> Temperature and aeration in the tank is set at optimal conditions.
>
> Can someone help identify the problem?
> Could anyone suggest alternative protocols for breeding?
>
> Thanks,
> Val
>
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Bill Trevarrow, Ph.D.
Thoren Aquatics
trevarro from uoneuro.uoregon.edu
Tel: (541) 844-9054
Fax: (541) 683-0996