[Zbrafish] RE: Zbrafish Digest, Vol 59, Issue 6

Guan, Qiang via zbrafish%40net.bio.net (by qiang.guan from Vanderbilt.Edu)
Thu Apr 8 13:56:20 EST 2010


To Val,

I agree that the light in your fish room might cause the fish stop laying eggs. We had the problem last Summer. Please check if the battery  in the timer was dead.


To Howie,

Do you clean  well your crossing cages after you used them?  I do. And  there should be no (or  a few) algae in  fish tanks, if you clean them every two weeks. The lifespan of male fish in our lab is longer than the female ones, because females have to lay many eggs. Keep the water clean in the system is our every day work.

Qiang  Guan
4/08

________________________________________
From: zbrafish-bounces from oat.bio.indiana.edu [zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of zbrafish-request from oat.bio.indiana.edu [zbrafish-request from oat.bio.indiana.edu]
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Subject: Zbrafish Digest, Vol 59, Issue 6

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Today's Topics:

   1. Dead Fish (Howie)
   2. RE: Zbrafish Digest, Vol 59, Issue 5 (Guan, Qiang)
   3. Re: Dead Fish (Angela)
   4. Re: Zebrafish females not looking gravid.contd (Daniel Castranova)


----------------------------------------------------------------------

Message: 1
Date: Wed, 7 Apr 2010 09:46:40 -0700 (PDT)
From: Howie <hsirotkin from sunysb.edu>
Subject: [Zbrafish] Dead Fish
To: bionet-organisms-zebrafish from moderators.isc.org
Message-ID:
        <2e656dfa-81a6-4596-a506-e823e73e241d from r1g2000yqb.googlegroups.com>
Content-Type: text/plain; charset=ISO-8859-1

Hi,

We've been losing fish when we set up crosses. The problem is sporadic
on some days we lose no fish, on others several. It is not a case of
aggression, as lethality occurs even when dividers separate the fish.
The overwhelming majority of the effected fish are male. Prior to
dying the fish swim sideways (as if tricained)  and can sometimes be
rescued by a change of water.  The fish in the system appear fine and
the general water quality parameters are in line, but we have recently
had more algae growth then usual in the system.  Ideas? Similar
experiences?

Thanks,

Howie



------------------------------

Message: 2
Date: Wed, 7 Apr 2010 14:29:08 -0500
From: "Guan, Qiang" <qiang.guan from Vanderbilt.Edu>
Subject: [Zbrafish] RE: Zbrafish Digest, Vol 59, Issue 5
To: "zbrafish from oat.bio.indiana.edu" <zbrafish from oat.bio.indiana.edu>
Message-ID:
        <4CBC119547951E4FBD63F68C97DE16630A09FC2B from ITS-HCWNEM03.ds.Vanderbilt.edu>

Content-Type: text/plain; charset="us-ascii"

Hi there,

I think that it is better to get some new young wild type  fish somewhere in your University, if it is available(if your female fish is not chubby at all). I have met the same problem before. I  will make the decision when the fish are three months old. If there were few female fish at that age, I will cross the adult fish again.  The selection of healthy fish embryos are also very important. Like human beings, the healthy parents will have the healthy young children... Could you tell me how much does it cost for your Artemia in 16 oz? We use Artemia from 40.00 - 90.00 USD per can. You have put too much variety of food in your fish, I think. The good wild type fish can lay eggs in the morning if you put  them into the crossing cage in the afternoon or lay eggs in the afternoon, if you put them into the crossing cage in the morning.
Do you have UV light in your fish water system?  Put fish into salty water.. I don't think that will help your fish..

________________________________________
From: zbrafish-bounces from oat.bio.indiana.edu [zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of zbrafish-request from oat.bio.indiana.edu [zbrafish-request from oat.bio.indiana.edu]
Sent: Wednesday, April 07, 2010 12:03 PM
To: zbrafish from magpie.bio.indiana.edu
Subject: Zbrafish Digest, Vol 59, Issue 5

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Today's Topics:

   1. Re: Zebrafish females not looking gravid (Bill Trevarrow)
   2. RE: Zbrafish Digest, Vol 59, Issue 4 (Guan, Qiang)
   3. Zebrafish females not looking gravid.contd (val)


----------------------------------------------------------------------

Message: 1
Date: Tue, 6 Apr 2010 10:49:34 -0700
From: Bill Trevarrow <trevarro from uoneuro.uoregon.edu>
Subject: Re: [Zbrafish] Zebrafish females not looking gravid
To: bionet-organisms-zebrafish from moderators.isc.org
Message-ID: <427C85FF-C752-4E81-A302-0345952FDF0D from uoneuro.uoregon.edu>
Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes

Hi Val,

You might want to check that you lights are going off at night.

Many automatic light systems have overrides that can accidentally be
set to keep the lights on all night. This will prevent the fish from
breeding and will not be obvious since normally no one is there when
the lights are supposed to be off.

On Apr 5, 2010, at 10:23 PM, val wrote:

> hi
> The adult zebrafish females in our facility do not seem to be getting
> 'chubby'.It is a problem indentifying the females to set them up for
> breeding. We have been feeding them about 3-4 times a day with variety
> of diets including Artemia,micropellets,Daphnia and tubifex worms.
>
> Moreover the fish have stopped breeding and there has been no egg
> production for the last 6 months. They show no sign of infection or
> disease. It is hard to tell if there is any chasing in the breeding
> tanks at all. The male:female ratio in the breeding tanks is 2:1. We
> have also setup mass breeding (4females+ 8males) in the tanks but to
> no success.
> We have been treating them with salt water once every week for the
> past 3 weeks to rule out any chance of infection. We tested the water
> for all relevant parameters and the water condition seems optimal.
> Temperature and aeration in the tank is set at optimal conditions.
>
> Can someone help identify the problem?
> Could anyone suggest alternative protocols for breeding?
>
> Thanks,
> Val
>
> _______________________________________________
> Zbrafish mailing list
> Zbrafish from net.bio.net
> http://www.bio.net/biomail/listinfo/zbrafish

Bill Trevarrow, Ph.D.
Thoren Aquatics
trevarro from uoneuro.uoregon.edu
Tel: (541) 844-9054
Fax: (541) 683-0996



------------------------------

Message: 2
Date: Tue, 6 Apr 2010 14:18:23 -0500
From: "Guan, Qiang" <qiang.guan from Vanderbilt.Edu>
Subject: [Zbrafish] RE: Zbrafish Digest, Vol 59, Issue 4
To: "zbrafish from oat.bio.indiana.edu" <zbrafish from oat.bio.indiana.edu>
Message-ID:
        <4CBC119547951E4FBD63F68C97DE16630A09FC2A from ITS-HCWNEM03.ds.Vanderbilt.edu>

Content-Type: text/plain; charset="us-ascii"

Hi there,

You didn't say how old your zebrafish was and what kind of fish. The wild type fish is easy to be taken care of. On the contrary,  tg and mutant fish are difficult to be raised up in good ratio(2 males : 1 famle is good).
The best time for adult fish breeding is 4 -12 months. It is waist time if your fish is over 15 months. If you have too many kinds of food to feed fish each day, do the fish overwight?

You should check which fish line lay eggs all the time and follow the way to handle your fish.

Qiang
4/06/2010

________________________________________
From: zbrafish-bounces from oat.bio.indiana.edu [zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of zbrafish-request from oat.bio.indiana.edu [zbrafish-request from oat.bio.indiana.edu]
Sent: Tuesday, April 06, 2010 12:07 PM
To: zbrafish from magpie.bio.indiana.edu
Subject: Zbrafish Digest, Vol 59, Issue 4

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        zbrafish from net.bio.net

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Today's Topics:

   1. Zebrafish females not looking gravid (val)


----------------------------------------------------------------------

Message: 1
Date: Mon, 5 Apr 2010 22:23:20 -0700 (PDT)
From: val <valetta.saldanha from gmail.com>
Subject: [Zbrafish] Zebrafish females not looking gravid
To: bionet-organisms-zebrafish from moderators.isc.org
Message-ID:
        <611b820a-1440-439c-a917-36c4ea2605fc from b33g2000yqc.googlegroups.com>
Content-Type: text/plain; charset=ISO-8859-1

hi
The adult zebrafish females in our facility do not seem to be getting
'chubby'.It is a problem indentifying the females to set them up for
breeding. We have been feeding them about 3-4 times a day with variety
of diets including Artemia,micropellets,Daphnia and tubifex worms.

Moreover the fish have stopped breeding and there has been no egg
production for the last 6 months. They show no sign of infection or
disease. It is hard to tell if there is any chasing in the breeding
tanks at all. The male:female ratio in the breeding tanks is 2:1. We
have also setup mass breeding (4females+ 8males) in the tanks but to
no success.
We have been treating them with salt water once every week for the
past 3 weeks to rule out any chance of infection. We tested the water
for all relevant parameters and the water condition seems optimal.
Temperature and aeration in the tank is set at optimal conditions.

Can someone help identify the problem?
Could anyone suggest alternative protocols for breeding?

Thanks,
Val



------------------------------

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End of Zbrafish Digest, Vol 59, Issue 4
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------------------------------

Message: 3
Date: Tue, 6 Apr 2010 23:59:01 -0700 (PDT)
From: val <valetta.saldanha from gmail.com>
Subject: [Zbrafish] Zebrafish females not looking gravid.contd
To: bionet-organisms-zebrafish from moderators.isc.org
Message-ID:
        <1047aec8-7f4d-4684-b244-2e3e9b82fa74 from z6g2000yqz.googlegroups.com>
Content-Type: text/plain; charset=ISO-8859-1

hi
Sincere thanks to those who responded to my previous post.

Adding details to my previous post (Zebrafish females not looking
gravid)..
We are trying to breed zebrafish (wild type) that are around 5 months
old. Simultaneously we are also putting the older fish (9-10 month
old) for breeding. They are housed in separate tanks. However the
younger/older females dont seem to be getting chubby. The sex ratio in
the tanks is about 1:1 male:female. The fish are not overweight or
lethargic. They show a healthy color and are active.Their diet is
wholesome with protein rich supplements.
We require a steady number of embryos each week.

Could anyone help me place where we are going wrong?



------------------------------

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End of Zbrafish Digest, Vol 59, Issue 5
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------------------------------

Message: 3
Date: Thu, 8 Apr 2010 05:40:13 -0700 (PDT)
From: Angela <busta.angela from gmail.com>
Subject: [Zbrafish] Re: Dead Fish
To: bionet-organisms-zebrafish from moderators.isc.org
Message-ID:
        <ddf5cb3e-8d88-44f0-bb90-25b5efff0e6c from b33g2000yqc.googlegroups.com>
Content-Type: text/plain; charset=ISO-8859-1

On Apr 7, 12:46?pm, Howie <hsirot... from sunysb.edu> wrote:
> Hi,
>
> We've been losing fish when we set up crosses. The problem is sporadic
> on some days we lose no fish, on others several. It is not a case of
> aggression, as lethality occurs even when dividers separate the fish.
> The overwhelming majority of the effected fish are male. Prior to
> dying the fish swim sideways (as if tricained) ?and can sometimes be
> rescued by a change of water. ?The fish in the system appear fine and
> the general water quality parameters are in line, but we have recently
> had more algae growth then usual in the system. ?Ideas? Similar
> experiences?
>
> Thanks,
>
> Howie

I had all the fish I set up die one day, a couple years ago. We were
never really sure shy but we were concerned about the hose that
connected to the fish system. the fish in the system were fine. we
started flushing the water out of the hose before using it to fill
breeding tanks and we did not have the problem again.



------------------------------

Message: 4
Date: Thu, 8 Apr 2010 08:47:52 -0400
From: Daniel Castranova <castrand from mail.nih.gov>
Subject: Re: [Zbrafish] Zebrafish females not looking gravid.contd
To: val <valetta.saldanha from gmail.com>
Cc: "bionet-organisms-zebrafish from moderators.isc.org"
        <bionet-organisms-zebrafish from moderators.isc.org>
Message-ID: <5010FCCD-21D7-427C-8FB4-24CE7115B957 from mail.nih.gov>
Content-Type: text/plain; charset=US-ASCII; format=flowed; delsp=yes

Val,


You mentioned it is hard to identify females.  Maybe you don't have
any females.  There is a chance that the sex ratio in your newest
generation of fish is heavily skewed towards male.  Although there are
many possible reasons for your fish not spawning I think that it boils
down to two possibilities:

1.  There is a problem with the fish (too inbred, all males, etc.)
2.  There is a problem with the husbandry (water quality, nutrition,
low level stressor, etc.)

To narrow down your problem I suggest that you order some adult fish
from ZIRC or get some from another institute that is near by.  If the
new fish spawn then the problem is with the fish.  If they don't, then
you have  a husbandry problem.


Hope that helps,

Dan




Daniel Castranova
Aquatic Specialist
Charles River Laboratories - Contractor
Unit on Vertebrate Organogenesis
Laboratory of Molecular Genetics, NICHD, NIH
Building 6B, Room 322, 6 Center Drive
Bethesda, MD 20892
(301)594-0904
castrand from mail.nih.gov

<"`DDD><
                         <"`CCC><



On Apr 7, 2010, at 2:59 AM, val wrote:

> hi
> Sincere thanks to those who responded to my previous post.
>
> Adding details to my previous post (Zebrafish females not looking
> gravid)..
> We are trying to breed zebrafish (wild type) that are around 5 months
> old. Simultaneously we are also putting the older fish (9-10 month
> old) for breeding. They are housed in separate tanks. However the
> younger/older females dont seem to be getting chubby. The sex ratio in
> the tanks is about 1:1 male:female. The fish are not overweight or
> lethargic. They show a healthy color and are active.Their diet is
> wholesome with protein rich supplements.
> We require a steady number of embryos each week.
>
> Could anyone help me place where we are going wrong?
>
> _______________________________________________
> Zbrafish mailing list
> Zbrafish from net.bio.net
> http://www.bio.net/biomail/listinfo/zbrafish



------------------------------

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