[Zbrafish] Re: Fish breeding issues
(by rachelboyle142 from gmail.com)
Tue Jun 8 19:26:02 EST 2010
On Jun 7, 10:40 pm, "Shia, Alice K H" <a.s... from imperial.ac.uk> wrote:
> Dear all,
> We have some transgenics that are now 6 months old and refusing to breed. I had 4 embroyos from them a few weeks ago and nothing before or since. The females are gravid and we give them blood meal before breeding.
> Anyone have any tips or ideas please?
There are several key factors for breeding, the most important we find
to be light, water and feeding. Best results for us come from pairing
after the usual 2 or 3 feeds/day (artemia/dry feed), leaving paired
overnight (12 hours dark) then a water change when the lights come on
the next morning. Also we pair one male to two females, or 3 females
to 2 males in a larger tank. Outcrossing transgenics (breeding with
wildtype) is much easier than incrossing, if you can sort the progeny.
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