[Zbrafish] CMV promoter coupled transgene expression

San via zbrafish%40net.bio.net (by santhosh.karanth from gmail.com)
Tue Mar 9 00:42:50 EST 2010


We injected a CMV promoter coupled transgene using tol2 method. The
founders died after first breeding. When we bred F1, the F2 are
positive for the transgene by PCR. However we did not detected neither
RNA nor hnRNA of the transgene using RT-PCR. Did anybody had this
probelm before with CMV?

Thanks.
Santhosh.



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