[Zbrafish] fixation of early stages

megha via zbrafish%40net.bio.net (by downandburied from gmail.com)
Fri Dec 9 22:39:59 EST 2011

Hi all !
I have a small problem ! when i fix my 6 and 12 somite stage
with 4%PFA overnight at 4degrees, the yolk sac becomes dark brown
i look it under the microscope the next day. I was  them in PBT (PBS+
Tween) and store in 100% MeOH for ISH. The embryo however, remains
clear. Can anyone please tell me why this happens and how can I
prevent it ? I have checked my PFA solution, and it seems to be fine.
please help !

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