[Zbrafish] RNA extraction from fixed samples
hannah.rouse.09 from ucl.ac.uk
(by hannah.rouse.09 from ucl.ac.uk)
Thu Dec 15 07:49:03 EST 2011
I would like to do qPCR and a microarray from zebrafish brains, but I
need to fix them before RNA extraction and purification. Has anyone
done this, and what sort of yield did you get from it?
Invitrogen has a nucleic acid isolation kit, which can purify from
fixed samples that have been in paraffin, http://products.invitrogen.com/ivgn/product/AM1975
and I am thinking of using this, without the deparaffinization step,
but if anyone has any prior experience of this, or a better/different
way of isolating the RNA, please let me know!!
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