[Zbrafish] Re: Wildtypes?

Burdine, Rebecca D via zbrafish%40net.bio.net (by rburdine from Princeton.EDU)
Tue Jan 4 14:28:02 EST 2011

We use RO water in our facility and add salts and bicarb to make system water.  Our RO water system for the building needed servicing.  We had a policy in place where they first shut off the lines to the building and the fish room BEFORE servicing.  But they forgot to shut off the lines to the fish rooms.  One of our rooms was flooded with bleach and ozone.  Not compatible for fish health and happiness if you were curious.

Thankfully our other room (with all of my mutant strains) had a reservoir before the tanks and we caught this before the cleaning solution hit the tanks.  We lost thousands of fish, but mainly wildtype and babies.

Now we have a sensor on our RO supply line that shuts off the line if it detects anything in the RO above set ppm cutoff.

Thankfully, ZIRC had us up a running in just a few months.


Rebecca D. Burdine, Ph.D.
Assistant Professor
Dept. of Molecular Biology
Princeton University
Washington Road Mof 433
Princeton, NJ 08544 
Phone: (609) 258-7515
Fax: (609) 258-6730
Email: rburdine from princeton.edu
Admin Assistant: Anna Schmedel (609) 258-5028

> -----Original Message-----
> From: zbrafish-bounces from oat.bio.indiana.edu [mailto:zbrafish-
> bounces from oat.bio.indiana.edu] On Behalf Of Jocelyn
> Sent: Tuesday, January 04, 2011 11:49 AM
> To: bionet-organisms-zebrafish from moderators.isc.org
> Subject: [Zbrafish] Re: Wildtypes?
> On Jan 3, 1:49 pm, "Burdine, Rebecca D" <rburd... from Princeton.EDU>
> wrote:
> > Background - we restored all our wildtypes with ZIRC stocks after mishap killed
> all our strains.  We invigorated them once with strains from the Schier lab.  We'd
> like to breed in another set of strains and then go back to ZIRC for the next round.
> thread hijack here...
> for posterity's sake, could you tell us in what the mishap was that
> killed off all your strains?
> good luck with your wt collection (we don't use any of those wt
> strains)
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