[Zbrafish] squeezing females
(by marie.winandy from gmail.com)
Mon Jan 10 03:33:37 EST 2011
Since several weeks I make in vitro fecondation of zebrafish as learned
at the ZIRC.
This week I was not successfull at all in squeezing females. Only 2 in
25 gave eggs, and the clutches were poor or mixed quality. Most of time
they didn't release any egg, and did not recover well from anesthesia.
I keep the females away from the males 1 or 2 days before squeezing.
All females from this group had normal shape and behaviour, were less
than 1 year old and looked healthy. I'm pretty sure that if I'd made
natural fertilisation, at least one third would have given eggs.
Does someone in the list have a tip to increase egg release, either in
conditionning the fish, diet, or handling, ....?
Thank you very much in advance
GIGA Research Institute
Universite de Liege, Belgium
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