[Zbrafish] squeezing females

[Steven Waldron]

I find it is helpful to breed the to-be-squeezed females a week in 
advance of the procedure- this helps to ensure the eggs will be of good 
quality.  I also set the female up in a divided breeding cage with a 
male the night before- perhaps the amorous advances of the male helps 
ease the flow of eggs?

best regards,
Steve Waldron
University of Washington, Seattle

Marie Winandy wrote:
> Hi all, 
>
> Since several weeks I make in vitro fecondation of zebrafish as learned
> at the ZIRC. 
>
> This week I was not successfull at all in squeezing females. Only 2 in
> 25 gave eggs, and the clutches were poor or mixed quality. Most of time
> they didn't release any egg, and did not recover well from anesthesia.
> I keep the females away from the males 1 or 2 days before squeezing.
> All females from this group had normal shape and behaviour, were less
> than 1 year old and looked healthy. I'm pretty sure that if I'd made
> natural fertilisation, at least one third would have given eggs. 
>
> Does someone in the list have a tip to increase egg release, either in
> conditionning the fish, diet, or handling, ....? 
>
> Thank you very much in advance
>
> Marie Winandy
> GIGA Research Institute
> Universite de Liege, Belgium
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