[Zbrafish] Re: Zebrafish facility problem - underdevelopment of embryos

[Steven Waldron]

You can stunt their development if the incubation dishes are overcrowded 
(no more than 50 embryos/dish).

best,
Steve Waldron
University of Washington, Seattle


>
> Hello Rachel,
>
> Thank you for advice. Unfortunately I do not think that the
> temperature have anything to do with our problem. Embryos are kept in
> 28C, in small volumes of E3 medium (about 10-15ml). Also the delay is
> quite big (at 24hpf they are in about 15 somite stage).
> Could you pleas write more about 3-4ppt solution you have mentioned?
> We are using methylene blue solution.
>
> Best regards
> Beata Filipek-Gorniok
>
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