[Zbrafish] Re: Zebrafish facility problem - underdevelopment of embryos

Steven Waldron via zbrafish%40net.bio.net (by waldrons from u.washington.edu)
Tue Jan 11 14:43:37 EST 2011

You can stunt their development if the incubation dishes are overcrowded 
(no more than 50 embryos/dish).

Steve Waldron
University of Washington, Seattle

> Hello Rachel,
> Thank you for advice. Unfortunately I do not think that the
> temperature have anything to do with our problem. Embryos are kept in
> 28C, in small volumes of E3 medium (about 10-15ml). Also the delay is
> quite big (at 24hpf they are in about 15 somite stage).
> Could you pleas write more about 3-4ppt solution you have mentioned?
> We are using methylene blue solution.
> Best regards
> Beata Filipek-Gorniok
> _______________________________________________
> Zbrafish mailing list
> Zbrafish from net.bio.net
> http://www.bio.net/biomail/listinfo/zbrafish

More information about the Zbrafish mailing list