[Zbrafish] Back once more for IACUC help

Michael Lardelli via zbrafish%40net.bio.net (by michael.lardelli from adelaide.edu.au)
Thu Mar 3 18:38:12 EST 2011

Fish must also experience stress merely though handling of tanks (e.g.
changes in light intensity coming from the surface below them) and will be
stressed by netting for placement in a tricaine solution etc. One advantage
of the ice slurry method is that it can be performed so rapidly with minimum
handling beforehand. At least that is one of the arguments I have put to our
Animal Ethics Committee. Regards, Michael. 


Michael Lardelli
Zebrafish Genetics Laboratory
School of Molecular and Biomedical Science The University of Adelaide,
Ph    : +61 8 8303 3212
Fax   : +61 8 8303 4362
e-mail: michael.lardelli from adelaide.edu.au CRICOS Provider Number 00123M
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From: zbrafish-bounces from oat.bio.indiana.edu
[mailto:zbrafish-bounces from oat.bio.indiana.edu] On Behalf Of Marie Winandy
Sent: Thursday, 3 March 2011 11:29 PM
To: zbrafish from oat.bio.indiana.edu
Subject: [Zbrafish] Back once more for IACUC help


Hi all, 


We use to euthanize our zebrafish with tricaine overdose (4g/L), and it is
really fast. 


I'm pretty sure that if the fish experience acute stress, it's during a very
short time (some 20 seconds at the maximum), thus far shorter than "rapid"
cooling. I've never seen a fish waking up from this concetrated tricaine


All the best, 


Marie Winandy PhD

Universite de Liege, Belgium

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