[Zbrafish] dechorionating embryos
(by eriksanders from wustl.edu)
Mon Sep 19 16:10:34 EST 2011
You can manually dechorionate using two pair of very fine forceps, or use a
chemical method that takes a little practice but is vastly superior when you
need to deal with lots of embryos. See below SOP.
The bleach treatment makes the chorion harder and the embryos will not be
able to hatch on their own. Therefore they must be manually dechorionated
with forceps, or with pronase .
Pronase 20mg/ml (Sigma P-5147)
1) add 1mL of Pronase to a Petri dish of up to 250 embryos (in egg water)
for exactly 3 minutes
2) pour off Pronase containing egg water and refill with autoclaved egg
water. Repeat this step for a total of 3 washouts.
3) Refill dish with autoclaved egg water.
Some embryos will have hatched immediately, others may require 24-hrs to
hatch, or can be hatched by gently swirling or pipetting up and down with a
> Can anyone tell me the easiest way of dechorionating early stage
> zebrafish embryos without disrupting the yolk?
> Thanks in advance,
> With Best wishes
> Zbrafish mailing list
> Zbrafish from net.bio.net
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