[Zbrafish] in situ hybridization : using early stages
(by sahartawakoli from yahoo.com)
Tue Sep 20 21:40:45 EST 2011
You may dechorionate the embryos both before and after fixation. Before 18 hpf it is better to do after fixation by using a pair of foreceps.
--- On Tue, 9/20/11, megha <downandburied from gmail.com> wrote:
From: megha <downandburied from gmail.com>
Subject: [Zbrafish] in situ hybridization : using early stages
To: bionet-organisms-zebrafish from moderators.isc.org
Date: Tuesday, September 20, 2011, 10:27 PM
when I do in situ hybridization on early stages of embryos (50%
epiboly etc) , then in situ staining gets trapped in the chorion. Can
i remove the chorion before fixing the embryos in PFA or after fixing
please help !
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