[Zbrafish] Re: food restriction

Devoto, Stephen via zbrafish%40net.bio.net (by sdevoto from wesleyan.edu)
Mon May 21 12:26:30 EST 2012


Michael:
I agree. Animal care in general, and food restriction in particular, are
not just appropriate topics, they are at the core of what I look for
zebrafish listserv.  It's is the only one that everybody working on
zebrafish is guaranteed to find relevant!

I do not know any of the animal care literature on whether fish (or other
ectotherms) experience pain and suffering from food restriction. One
positive health effect of food restriction could come from an improvement
in water quality.  From my aquaria at home, I can report that over-feeding
is a much greater fish welfare problem than not feeding. Fish at my house
(not covered by an animal care and use protocol!) have gone months without
food; if anything this seemed to make them more robust. On the other hand,
when we had a neighbor stop by to feed the fish and the cat for a week,
most fish died, and the ones that survived her over-feeding were
grotesquely distended.

Stephen
-----------------------
Stephen H. Devoto, Associate Professor
Biology, Neuroscience and Behavior
Wesleyan University, Middletown, CT  06459
office: (860) 685-3461	Lab: (860) 685-3206
http://sdevoto.web.wesleyan.edu







On 5/21/12 1:03 PM, "zbrafish-request from oat.bio.indiana.edu"
<zbrafish-request from oat.bio.indiana.edu> wrote:

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>Today's Topics:
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>   1. Re: RE: Starvation of older zebrafish to increase egg
>      production? (Steven Waldron)
>   2. Re: eGFP is detected in red channel confocal (Sahar Tavakoli)
>
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>----------------------------------------------------------------------
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>Message: 1
>Date: Sat, 19 May 2012 10:27:41 -0700
>From: Steven Waldron <stevenwaldron from gmail.com>
>Subject: Re: [Zbrafish] RE: Starvation of older zebrafish to increase
>	egg	production?
>To: "Burdine, Rebecca D." <rburdine from princeton.edu>
>Cc: "bionet-organisms-zebrafish from moderators.isc.org"
>	<bionet-organisms-zebrafish from moderators.isc.org>
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>You might also try separating the males from females for at least a week,
>increase the frequency of water changes, and most importantly... feed a
>diet heavy in Artemia nauplii.
>
>Breeding success can start to become less predictable once a fish reaches
>its first birthday.
>
>I've never tried the starvation trick, never really had reason to...but I
>do wonder if it might mimic some seasonal variation in food supply
>availability and spawning frequency (e.g. dry season vs. monsoon)?
>
>
>
>On Fri, May 18, 2012 at 3:25 PM, Burdine, Rebecca D.
><rburdine from princeton.edu
>> wrote:
>
>>
>> But we have some strains that just give out at 1 year no matter what we
>> do, and others that still produce reasonably ok at 3.  I am happy to
>>share
>> our feeding protocols with you if you like.  They are essentially
>>modified
>> protocols that are used at ZIRC.
>>
>> Becky
>> ________________________________________
>> From: zbrafish-bounces from oat.bio.indiana.edu [
>> zbrafish-bounces from oat.bio.indiana.edu] on behalf of David G. White [
>> dgw5079 from u.washington.edu]
>> Sent: Thursday, May 17, 2012 8:15 PM
>> To: Michael Lardelli
>> Cc: bionet-organisms-zebrafish from moderators.isc.org
>> Subject: Re: [Zbrafish] RE: Starvation of older zebrafish to increase
>>egg
>>       production?
>>
>> If your following proper animal laboratory protocols you would not have
>> the problem in the first place. Your fish may already be under stress
>>and
>> you seek knowledge to possibly exacerbate your situation! Perhaps
>>culling
>> the fish after 12 months is better than 18, either way we do neither
>>here,
>> our fish are reliable breeders up to 2 years.
>>
>> I must admit I was a bit shocked when I saw your subject and I apologize
>> if you took offense to my comments. We are all looking for knowledge
>>but I
>> don't think your subject is proper for this forum.
>>
>> David G White
>> Research Coordinator
>> HSB Zebrafish Labs
>> University of Washington
>> Department of Biological Structure
>> HSB G520 Box 357420
>> 1959 NE Pacific Street
>> Seattle, WA  98195-7420
>> Tel. 206-685-7512
>> FAX 206-543-1524
>>
>> On Thu, 17 May 2012, Michael Lardelli wrote:
>>
>> >
>> > I am not sure which is more inhumane ? starving fish to lengthen their
>> productive life or
>> > culling them after 18 months because they have become unreliable
>> spawners. Culling could
>> > certainly be regarded as a ?short cut?! In any case, I am still
>> interested to hear from anyone
>> > who has knowledge or experience in this area.
>> >
>> >
>> >
>> > Regards,
>> >
>> >
>> >
>> > Michael
>> >
>> >
>> >
>> > Michael Lardelli
>> > Zebrafish Genetics Laboratory
>> > School of Molecular and Biomedical Science The University of Adelaide,
>> AUSTRALIA 5005
>> > Ph    : +61 8 8303 3212
>> > Fax   : +61 8 8303 4362
>> > e-mail: michael.lardelli from adelaide.edu.au CRICOS Provider Number 00123M
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>Message: 2
>Date: Fri, 18 May 2012 19:22:49 -0700 (PDT)
>From: Sahar Tavakoli <sahartawakoli from yahoo.com>
>Subject: Re: [Zbrafish] eGFP is detected in red channel confocal
>To: Wei <ilovequestions99 from gmail.com>,
>	"Zbrafish from magpie.bio.indiana.edu" <Zbrafish from magpie.bio.indiana.edu>
>Message-ID:
>	<1337394169.41942.YahooMailNeo from web126006.mail.ne1.yahoo.com>
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>
>Hi Lucas,
>
>It could be noise, as weak signals and the other FP are?co-localized. Do
>you use the separate filter for each channel. E. g when you are imaging
>for GFP change the?excitation?filter for GFP excitation?wavelength. So,
>any other?wavelengths?except GFP would be omitted. And the same for
>dTomate.
>
>Good luck
>
>Sahar
>
>
>________________________________
> From: Wei <ilovequestions99 from gmail.com>
>To: Zbrafish from magpie.bio.indiana.edu
>Sent: Friday, May 18, 2012 11:41 AM
>Subject: [Zbrafish] eGFP is detected in red channel confocal
> 
>
>Hello, I would like to know if anyone have similar experiences to share?
>In eGFP and Tdtomato double transgenic zebrafish larvae (12 dpf from two
>different promoters from different genes), eGFP was always detected
>weakly in the red channel but not vice versa. It looks like eGFP
>expressing cells are also expressing Tdtomato weakly. Tried to do ICC by
>double antibody staining but could not find good antibody against
>Tdtomato. Please share with me too if you have good experience with
>Tdtomato antibody (manufacturer or catalog#?).
>
>Thank you so much for your sharing!
>
>Lucas
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