[Zbrafish] Re: food restriction

Steven Waldron via zbrafish%40net.bio.net (by stevenwaldron from gmail.com)
Mon May 21 20:08:40 EST 2012


In general, a small freshwater tropical fish with the metabolism and
activity of a zebrafish can go around two weeks without food.  I also find
these starvation periods will test the strength of the fish well-- the weak
usually do not fare so well.

Note:  these are observations collected from my home aquaria!  Even
dedicated fish geeks have to go on vacation at times.

On Mon, May 21, 2012 at 10:26 AM, Devoto, Stephen <sdevoto from wesleyan.edu>wrote:

> Michael:
> I agree. Animal care in general, and food restriction in particular, are
> not just appropriate topics, they are at the core of what I look for
> zebrafish listserv.  It's is the only one that everybody working on
> zebrafish is guaranteed to find relevant!
>
> I do not know any of the animal care literature on whether fish (or other
> ectotherms) experience pain and suffering from food restriction. One
> positive health effect of food restriction could come from an improvement
> in water quality.  From my aquaria at home, I can report that over-feeding
> is a much greater fish welfare problem than not feeding. Fish at my house
> (not covered by an animal care and use protocol!) have gone months without
> food; if anything this seemed to make them more robust. On the other hand,
> when we had a neighbor stop by to feed the fish and the cat for a week,
> most fish died, and the ones that survived her over-feeding were
> grotesquely distended.
>
> Stephen
> -----------------------
> Stephen H. Devoto, Associate Professor
> Biology, Neuroscience and Behavior
> Wesleyan University, Middletown, CT  06459
> office: (860) 685-3461        Lab: (860) 685-3206
> http://sdevoto.web.wesleyan.edu
>
>
>
>
>
>
>
> On 5/21/12 1:03 PM, "zbrafish-request from oat.bio.indiana.edu"
> <zbrafish-request from oat.bio.indiana.edu> wrote:
>
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> >Today's Topics:
> >
> >   1. Re: RE: Starvation of older zebrafish to increase egg
> >      production? (Steven Waldron)
> >   2. Re: eGFP is detected in red channel confocal (Sahar Tavakoli)
> >
> >
> >----------------------------------------------------------------------
> >
> >Message: 1
> >Date: Sat, 19 May 2012 10:27:41 -0700
> >From: Steven Waldron <stevenwaldron from gmail.com>
> >Subject: Re: [Zbrafish] RE: Starvation of older zebrafish to increase
> >       egg     production?
> >To: "Burdine, Rebecca D." <rburdine from princeton.edu>
> >Cc: "bionet-organisms-zebrafish from moderators.isc.org"
> >       <bionet-organisms-zebrafish from moderators.isc.org>
> >Message-ID:
> >       <
> CAP2Q5346gUUNzrk6rr263DjzbgeGn3nOmKusX1_5Co9fmjVqrg from mail.gmail.com>
> >Content-Type: text/plain; charset="windows-1252"
> >
> >You might also try separating the males from females for at least a week,
> >increase the frequency of water changes, and most importantly... feed a
> >diet heavy in Artemia nauplii.
> >
> >Breeding success can start to become less predictable once a fish reaches
> >its first birthday.
> >
> >I've never tried the starvation trick, never really had reason to...but I
> >do wonder if it might mimic some seasonal variation in food supply
> >availability and spawning frequency (e.g. dry season vs. monsoon)?
> >
> >
> >
> >On Fri, May 18, 2012 at 3:25 PM, Burdine, Rebecca D.
> ><rburdine from princeton.edu
> >> wrote:
> >
> >>
> >> But we have some strains that just give out at 1 year no matter what we
> >> do, and others that still produce reasonably ok at 3.  I am happy to
> >>share
> >> our feeding protocols with you if you like.  They are essentially
> >>modified
> >> protocols that are used at ZIRC.
> >>
> >> Becky
> >> ________________________________________
> >> From: zbrafish-bounces from oat.bio.indiana.edu [
> >> zbrafish-bounces from oat.bio.indiana.edu] on behalf of David G. White [
> >> dgw5079 from u.washington.edu]
> >> Sent: Thursday, May 17, 2012 8:15 PM
> >> To: Michael Lardelli
> >> Cc: bionet-organisms-zebrafish from moderators.isc.org
> >> Subject: Re: [Zbrafish] RE: Starvation of older zebrafish to increase
> >>egg
> >>       production?
> >>
> >> If your following proper animal laboratory protocols you would not have
> >> the problem in the first place. Your fish may already be under stress
> >>and
> >> you seek knowledge to possibly exacerbate your situation! Perhaps
> >>culling
> >> the fish after 12 months is better than 18, either way we do neither
> >>here,
> >> our fish are reliable breeders up to 2 years.
> >>
> >> I must admit I was a bit shocked when I saw your subject and I apologize
> >> if you took offense to my comments. We are all looking for knowledge
> >>but I
> >> don't think your subject is proper for this forum.
> >>
> >> David G White
> >> Research Coordinator
> >> HSB Zebrafish Labs
> >> University of Washington
> >> Department of Biological Structure
> >> HSB G520 Box 357420
> >> 1959 NE Pacific Street
> >> Seattle, WA  98195-7420
> >> Tel. 206-685-7512
> >> FAX 206-543-1524
> >>
> >> On Thu, 17 May 2012, Michael Lardelli wrote:
> >>
> >> >
> >> > I am not sure which is more inhumane ? starving fish to lengthen their
> >> productive life or
> >> > culling them after 18 months because they have become unreliable
> >> spawners. Culling could
> >> > certainly be regarded as a ?short cut?! In any case, I am still
> >> interested to hear from anyone
> >> > who has knowledge or experience in this area.
> >> >
> >> >
> >> >
> >> > Regards,
> >> >
> >> >
> >> >
> >> > Michael
> >> >
> >> >
> >> >
> >> > Michael Lardelli
> >> > Zebrafish Genetics Laboratory
> >> > School of Molecular and Biomedical Science The University of Adelaide,
> >> AUSTRALIA 5005
> >> > Ph    : +61 8 8303 3212
> >> > Fax   : +61 8 8303 4362
> >> > e-mail: michael.lardelli from adelaide.edu.au CRICOS Provider Number
> 00123M
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> >>
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> >Message: 2
> >Date: Fri, 18 May 2012 19:22:49 -0700 (PDT)
> >From: Sahar Tavakoli <sahartawakoli from yahoo.com>
> >Subject: Re: [Zbrafish] eGFP is detected in red channel confocal
> >To: Wei <ilovequestions99 from gmail.com>,
> >       "Zbrafish from magpie.bio.indiana.edu" <Zbrafish from magpie.bio.indiana.edu
> >
> >Message-ID:
> >       <1337394169.41942.YahooMailNeo from web126006.mail.ne1.yahoo.com>
> >Content-Type: text/plain; charset="iso-8859-1"
> >
> >Hi Lucas,
> >
> >It could be noise, as weak signals and the other FP are?co-localized. Do
> >you use the separate filter for each channel. E. g when you are imaging
> >for GFP change the?excitation?filter for GFP excitation?wavelength. So,
> >any other?wavelengths?except GFP would be omitted. And the same for
> >dTomate.
> >
> >Good luck
> >
> >Sahar
> >
> >
> >________________________________
> > From: Wei <ilovequestions99 from gmail.com>
> >To: Zbrafish from magpie.bio.indiana.edu
> >Sent: Friday, May 18, 2012 11:41 AM
> >Subject: [Zbrafish] eGFP is detected in red channel confocal
> >
> >
> >Hello, I would like to know if anyone have similar experiences to share?
> >In eGFP and Tdtomato double transgenic zebrafish larvae (12 dpf from two
> >different promoters from different genes), eGFP was always detected
> >weakly in the red channel but not vice versa. It looks like eGFP
> >expressing cells are also expressing Tdtomato weakly. Tried to do ICC by
> >double antibody staining but could not find good antibody against
> >Tdtomato. Please share with me too if you have good experience with
> >Tdtomato antibody (manufacturer or catalog#?).
> >
> >Thank you so much for your sharing!
> >
> >Lucas
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