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Regarding RNAi in zebrafish, this might be helpful:<br>
<br>
<span class="authors">Gruber J, Manninga H, Tuschl T, Osborn M, Weber K.</span><span
class="icon"></span>
<div class="title">Specific RNAi mediated gene knockdown in zebrafish
cell lines.</div>
<div class="source">RNA Biol. 2005 Jul-Sep;2(3):101-5. Epub 2005 Jul 19.<br>
<br>
The paper includes data on work in embryos, but they do not claim
success in embryos with RNAi.<br>
Abstract:<br>
<a class="moz-txt-link-freetext" href="http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17114924">http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17114924</a><br>
Open-access paper:<br>
<a class="moz-txt-link-freetext" href="http://www.landesbioscience.com/journals/rnabiology/article/gruberRNA2-3.pdf">http://www.landesbioscience.com/journals/rnabiology/article/gruberRNA2-3.pdf</a><br>
<br>
Let me know how I can help.<br>
<br>
Regards,<br>
<br>
- Jon<br>
<br>
Jon D. Moulton, Ph.D.<br>
Diagnostics and Special Projects<br>
GENE TOOLS, LLC<br>
<a class="moz-txt-link-abbreviated" href="mailto:jmoulton@gene-tools.com">jmoulton@gene-tools.com</a><br>
<a class="moz-txt-link-abbreviated" href="http://www.gene-tools.com">www.gene-tools.com</a><br>
(541) 929-7840 x1201<br>
<a class="moz-txt-link-freetext" href="http://network.nature.com/group/morpholinos">http://network.nature.com/group/morpholinos</a><br>
<br>
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bdschyth wrote:
<blockquote
cite="mid:1195029061.855314.3400@d55g2000hsg.googlegroups.com"
type="cite">
<pre wrap="">On Sep 18, 4:29 am, Joule <a class="moz-txt-link-rfc2396E" href="mailto:bwj...@gmail.com"><bwj...@gmail.com></a> wrote:
</pre>
<blockquote type="cite">
<pre wrap="">On Jul 24, 11:58 pm, senthil kumar <a class="moz-txt-link-rfc2396E" href="mailto:rse...@yahoo.co.in"><rse...@yahoo.co.in></a> wrote:
</pre>
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<pre wrap="">Is it possible to do RNAi in Zebrafish. Please send me the references..
Thanks
Rsen
</pre>
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<pre wrap="">---------------------------------
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<pre wrap="">It seems that RNAi does not work in zebrafish .
</pre>
</blockquote>
<pre wrap=""><!---->-----------------------------------------------------------------------------------------------
But - why does it not work in fish? I dont think it is that well
investigated yet. Most studies have been in zebrafish embryos
microinjected with long dsRNAs. Remember that not many mammalian
studies on RNAi mediated gene silencing has been publiched neither.
Brian Dall Schyth, National Veterinary Institute, Denmark
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