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Hi Gabe,<div>It has been 10 years since I fought my battle against Coleps, and it was a tough battle to overcome. Here are my suggestions:</div><div><br class="webkit-block-placeholder"></div><div>1. Bleach all of your eggs around 24 hours (approx. 35ppm bleach - Zebrafish Book concentration).</div><div>2. Be sure to remove ALL dead/damaged eggs before bleaching (Coleps can hide and make their way through bleach in damaged eggs).</div><div>3. After bleaching, keep your eggs/embryos in embryo media until they have inflated their swim bladders and are swimming. Zebrafish larvae are the most susceptible when they have hatched, but not yet inflated their swim bladders. Once the larvae are able to swim properly, they can fend off the Coleps. </div><div><br class="webkit-block-placeholder"></div><div>Colpes are very hard to get rid of. I am unaware of any drugs that will kill them, but I am fairly sure that UV works. Bleaching and filtering our nursery water through a micron mesh helped us contain the problem years ago. When we built the Resource Center, we were lucky not to bring them with us. I continue to keep my fingers crossed that it stays that way.</div><div><br class="webkit-block-placeholder"></div><div>Good luck, and let me know if you have any questions.</div><div><br class="webkit-block-placeholder"></div><div>April</div><div><br class="webkit-block-placeholder"></div><div><br class="webkit-block-placeholder"></div><div><br><div><div>On Jan 31, 2008, at 5:47 PM, <a href="mailto:finchg@ohsu.edu">finchg@ohsu.edu</a> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">________________________________________________________________________________</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">I am looking for more information about coleps.<span class="Apple-converted-space"> </span>These are a small</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">protazoa which parasatize larval zebrafish.<span class="Apple-converted-space"> </span>Under a dissecting scope</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">they look like little transparent hyperactive beans, less than one</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">hundredth the size of a zebrafish egg, comparable to a zfish blood</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">cell in size.<span class="Apple-converted-space"> </span>I am only able to see them at >5X mag.</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">-Here's a little mention in an old Zebrafish Science Monitor:</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://zfin.org/zf_info/monitor/vol5.1/vol5.1.html#Coleps,%20Scourge%20of%20the%20Baby%20Zebrafish">http://zfin.org/zf_info/monitor/vol5.1/vol5.1.html#Coleps,%20Scourge%20of%20the%20Baby%20Zebrafish</a></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Three seperate zebrafish labs at which I have worked have had minor</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">problems with these little guys (Perhaps I am the vector, dunno).</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">-Does anyone know more about these things than what is stated in the</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">article linked above?<span class="Apple-converted-space"> </span>E.g. Life cycle, response to dessication, drugs</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">with which to kill them?</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">-Anyone had problems with them that they have overcome?<span class="Apple-converted-space"> </span>What</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">strategies worked?<span class="Apple-converted-space"> </span>So far the only good strategy I know of is</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">vigilant dish cleaning.</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">-We have lately been having problems in dishes which we have bleached,</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">which is perplexing.<span class="Apple-converted-space"> </span>Do other people see them surviving bleaching?</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Killed by bleaching?<span class="Apple-converted-space"> </span>(We follow the Volhard protocol)</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">-Some folks have suggested raising water conductivity in our system.</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Anyone agree?</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">-Also, does anyone know if UV can kill these or specifically what</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">levels?</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">-Anyone offering up advice or results regarding these pesky things</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">will be my personal hero.</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Thanks, Gabe</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">_______________________________________________</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Zbrafish mailing list</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="mailto:Zbrafish@net.bio.net">Zbrafish@net.bio.net</a></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.bio.net/biomail/listinfo/zbrafish">http://www.bio.net/biomail/listinfo/zbrafish</a></div> </blockquote></div><br><div> <span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Verdana; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">-----------------------</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">April R. Freeman (Mazanec)</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">ZIRC Manager</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Zebrafish Int.'l Resource Center</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">5274 University of Oregon</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Eugene, OR 97403-5274</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 12px/normal Verdana; min-height: 15px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Phone: (541) 346-6028 ext. 24</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Fax: (541) 346-6151</div><br class="Apple-interchange-newline"></span> </div><br></div></body></html>
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