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You can find some good antibodies from here: <a href="http://www.genscript.com/cgi-bin/products/rec_antibody.cgi">http://www.genscript.com/cgi-bin/products/rec_antibody.cgi</a> <div class="gmail_quote">On Thu, May 15, 2008 at 1:03 PM, <<a href="mailto:zbrafish-request@oat.bio.indiana.edu">zbrafish-request@oat.bio.indiana.edu</a>> wrote: <blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">Send Zbrafish mailing list submissions to        <a href="mailto:zbrafish@net.bio.net">zbrafish@net.bio.net</a> To subscribe or unsubscribe via the World Wide Web, visit        <a href="http://www.bio.net/biomail/listinfo/zbrafish" target="_blank">http://www.bio.net/biomail/listinfo/zbrafish</a> or, via email, send a message with subject or body 'help' to        <a href="mailto:zbrafish-request@net.bio.net">zbrafish-request@net.bio.net</a> You can reach the person managing the list at        <a href="mailto:zbrafish-owner@net.bio.net">zbrafish-owner@net.bio.net</a> When replying, please edit your Subject line so it is more specific than "Re: Contents of Zbrafish digest..." Today's Topics:   1. Measurement of compound uptake into the fish embryo (fran?ois)   2. IP3R Antibody for Zebrafish (<a href="mailto:chris.cyk@gmail.com">chris.cyk@gmail.com</a>)   3. IP3R Antibody for Zebrafish (Chris) ---------------------------------------------------------------------- Message: 1 Date: Wed, 14 May 2008 06:30:37 -0700 (PDT) From: fran?ois <<a href="mailto:busquet.francois@gmail.com">busquet.francois@gmail.com</a>> Subject: [Zbrafish] Measurement of compound uptake into the fish        embryo To: <a href="mailto:bionet-organisms-zebrafish@moderators.isc.org">bionet-organisms-zebrafish@moderators.isc.org</a> Message-ID:        <<a href="mailto:0e26645e-8da3-4a5a-86d3-ec49eb4eb801@r66g2000hsg.googlegroups.com">0e26645e-8da3-4a5a-86d3-ec49eb4eb801@r66g2000hsg.googlegroups.com</a>> Content-Type: text/plain; charset=ISO-8859-1 Hi, I am actually reviewing possible methods to measure compound uptake into the fish embryos. Until now, LC-MS protocols are available. However, I also heard about algorithms but I could not find any reference. I would be grateful if anybody knew about it or others methods. Regards, françois ------------------------------ Message: 2 Date: Tue, 13 May 2008 18:29:20 -0700 (PDT) From: <a href="mailto:chris.cyk@gmail.com">chris.cyk@gmail.com</a> Subject: [Zbrafish] IP3R Antibody for Zebrafish To: <a href="mailto:bionet-organisms-zebrafish@moderators.isc.org">bionet-organisms-zebrafish@moderators.isc.org</a> Message-ID:        <<a href="mailto:5cabaeb9-b680-4dd1-a0e8-bd3828bc8595@u12g2000prd.googlegroups.com">5cabaeb9-b680-4dd1-a0e8-bd3828bc8595@u12g2000prd.googlegroups.com</a>> Content-Type: text/plain; charset=ISO-8859-1 I'm looking for the antibody that labels inositol 1,4,5-trisphosphate receptors (IP3R) in zebrafish. I've tried several commerical available antibodies, but they're all not working. Do you know any IP3R antibody that is working in zebrafish? Thank you very much. Chris Cheung Graduate student Department of Biology The Hong Kong University of Science & Technology Clear Water Bay, Kowloon, Hong Kong Tel: (852) 2358-7324 Fax: (852) 2358-7323 ------------------------------ Message: 3 Date: Tue, 13 May 2008 18:50:57 -0700 (PDT) From: Chris <<a href="mailto:chris.cyk@gmail.com">chris.cyk@gmail.com</a>> Subject: [Zbrafish] IP3R Antibody for Zebrafish To: <a href="mailto:bionet-organisms-zebrafish@moderators.isc.org">bionet-organisms-zebrafish@moderators.isc.org</a> Message-ID:        <<a href="mailto:a47da2a2-358a-488b-b104-0d0a048f6ed3@p39g2000prm.googlegroups.com">a47da2a2-358a-488b-b104-0d0a048f6ed3@p39g2000prm.googlegroups.com</a>> Content-Type: text/plain; charset=ISO-8859-1 I'm looking for the antibody that labels inositol 1,4,5-trisphosphate receptors (IP3R) in zebrafish. I've tried several commerical available antibodies, but they're all not working. Do you know any IP3R antibody that is working in zebrafish? Thank you very much. Chris Cheung Graduate student Department of Biology The Hong Kong University of Science & Technology Clear Water Bay, Kowloon, Hong Kong Tel: (852) 2358-7324 Fax: (852) 2358-7323 ------------------------------ _______________________________________________ Zbrafish mailing list <a href="mailto:Zbrafish@net.bio.net">Zbrafish@net.bio.net</a> <a href="http://www.bio.net/biomail/listinfo/zbrafish" target="_blank">http://www.bio.net/biomail/listinfo/zbrafish</a> End of Zbrafish Digest, Vol 36, Issue 7 *************************************** </blockquote></div>

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