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<html> <head> <style> .hmmessage P { margin:0px; padding:0px } body.hmmessage { FONT-SIZE: 10pt; FONT-FAMILY:Tahoma } </style> </head> <body class='hmmessage'> Hi! Thank you for the article. I have an other question: to  avoid that blood coagulates, where do you put the sample after the extraction? I tried to use the haparin, but this anticoagulant stop the polymerase activity and I need to use the sample of blood to extract RNA and make PCR... > To: bionet-organisms-zebrafish@moderators.isc.org > From: claudia_hohn@hotmail.com > Subject: Re: FW: [Zbrafish] blood extraction > Date: Fri, 30 May 2008 09:42:26 -0700 > CC: > > On May 29, 6:00 am, maria chiara pelleri <maripell...@hotmail.com> > wrote: > > From: maripell...@hotmail.com > > To: zbraf...@magpie.bio.indiana.edu > > Subject: RE: [Zbrafish] blood extraction > > Date: Thu, 29 May 2008 09:16:34 +0000 > > > > Sorry! > > I didn't specify that I need to extract the greatest quantity of blood possible because I want to extract the RNA from the sample of blood, I usually kill the fish and I use adult fish. > > Can you help me? > > Thanks > > Maria Chiara > > > > Maria Chiara Pelleri > > University of Bologna, Italy > > > > From: maripell...@hotmail.com > > To: zbraf...@magpie.bio.indiana.edu > > Date: Wed, 28 May 2008 13:25:54 +0000 > > CC: > > Subject: [Zbrafish] blood extraction > > > > Hi! > > I'm searching the best method for extracting the blood from > > individual zebrafish. If you had any advice or particular methods for > > extracting the blood from zf, please, answer me!!! > > Thanks > > Maria Chiara Pelleri > > > > Windows Live Mail Controlla i tuoi account di posta con un unico programma, è GRATIS! > > > > Windows Live Mail Controlla i tuoi account di posta con un unico programma, è GRATIS! > > > > _________________________________________________________________ > > Scarica Windows Live, un mondo di programmi per te!http://get.live.com/ > > Hi Maria, > we use the method described by P. JAGADEESWARAN, J. P. SHEEHAN, F. E. > CRAIG > AND D. TROYER: Identification and characterization of zebrafish > thrombocytes. British Journal of Haematology, 1999, 107, 731-738. > Depending on the size of the zebrafish you get between 5-10ul of > blood. > Claudia > > _______________________________________________ > Zbrafish mailing list > Zbrafish@net.bio.net > http://www.bio.net/biomail/listinfo/zbrafish <hr />Windows Live Mobile <a href='http://windowslivemobile.msn.com/Homepage.aspx?lang=it-it ' target='_new'>Collegati a Messenger dal tuo cellulare!</a></body> </html>

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