<div dir="ltr">There's an article by Amali et al (Journal of Biomedical Science (2006) 13: 225–232) where they looked for apoptosis in liver cells of embryos using TUNEL method. After 7 days they observed a diminished number of apoptotic bodies, due to the increasing number of necrotic bodies. I hope it helps you.<br>
Regards,<br>Ana Schneider<br><br><br><br><div class="gmail_quote">On Fri, Jul 18, 2008 at 10:01 AM, Tyrone <<a href="mailto:tgenade@gmail.com">tgenade@gmail.com</a>> wrote:<br><blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">
Hello<br>
<br>
On Jul 16, 3:45 pm, stefan1306 <<a href="mailto:stefan.we...@merck.de">stefan.we...@merck.de</a>> wrote:<br>
<br>
> at the moment I´m dealing with histology of 96 hpf embryos and I want<br>
> to show necrosis...<br>
<br>
You may want to pose this question to the histonet group. They may be<br>
more forthcoming with useful information. You may also want to track<br>
down Maldonado et al Neurobiol. 53:21--35, 2002. In it they show<br>
neurodegeneration<br>
(via what appears to be a non-apoptotic mechanism) using cresyl<br>
violet.<br>
<br>
If you find a better means to show necrosis, please let me know! I am<br>
very far from being an expert on this topic.<br>
<br>
Kind regards<br>
<br>
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