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Hi Rachel,<div>To determine if these are in fact coleps, look at them under a compound microscope. Coleps are very easy to identify. They move in a swirling pattern and their body's are covered in plates that make them look like hand grenades. Unhatched or recently hatch embryos that have not developed swim bladders are the most susceptible to coleps. A swarm of coleps can consume an entire embryo within 45 minutes. Once embryos are swimming they can fend them off quite easily. If the parasites were only found in 1 embryo, I wouldn't worry to much yet. Keep your embryos as clean as possible and remove anything suspicious. You should also consider bleaching your embryos, if you are not currently.<div><br></div><div>I would be happy to take a look at some pictures and help identify the parasite. Good luck, and let me know if you have any questions.</div><div><br></div><div>April</div><div><br></div><div><div><br></div><div><div><div>On Jul 31, 2008, at 6:16 PM, <a href="mailto:RachelRaynes@gmail.com">RachelRaynes@gmail.com</a> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">While performing maintenance on a clutch of 48 hour zebrafish embryos,</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">I came across one with a swarm of parasites within its chorion.<span class="Apple-converted-space"> </span>All</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">the other embryos in the clutch were fine.<span class="Apple-converted-space"> </span>I examined the embryo 5</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">hours later and it was almost completely devoured.<span class="Apple-converted-space"> </span>I assume that this</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">is a parasite that the mother passed on?<span class="Apple-converted-space"> </span>Unfortunately, the cross was</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">albino stock and the individual fish was put back into a group tank</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">after breeding.<span class="Apple-converted-space"> </span>What were those things and what action if any needs</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">to be taken?</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">If anyone would like a picture or video of the embryo I’d be happy to</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">send one in hopes of identifying the parasite.</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">_______________________________________________</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Zbrafish mailing list</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="mailto:Zbrafish@net.bio.net">Zbrafish@net.bio.net</a></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.bio.net/biomail/listinfo/zbrafish">http://www.bio.net/biomail/listinfo/zbrafish</a></div> </blockquote></div><br><div> <span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Verdana; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">-----------------------</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">April R. Freeman (Mazanec)</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">ZIRC Manager</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Zebrafish Int.'l Resource Center</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">5274 University of Oregon</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Eugene, OR 97403-5274</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 12px/normal Verdana; min-height: 15px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Phone: (541) 346-6028 ext. 24</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Fax: (541) 346-6151</div><br class="Apple-interchange-newline"></span> </div><br></div></div></div></body></html>
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