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Regarding the question:<br>
"Hi, I just started to do microinjection. I am wondering how to
calibrate the needles so that I know the amount I am injecting."<br>
<br>
See the <a href="http://www.sciencemag.org/cgi/data/1142538/DC1/1">supporting
online material</a> of the following citation for <b>methods for
characterizing microinjected volume</b> per embryo. <br>
Riedel-Kruse IH, Muller C, Oates AC. <a
href="http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17702912">Synchrony
dynamics during initiation, failure, and rescue of the segmentation
clock.</a> Science. 2007 Sep 28;317(5846):1911-5. Epub 2007 Aug 16.<br>
<br>
<a class="moz-txt-link-freetext" href="http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17702912">http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=17702912</a><br>
<a class="moz-txt-link-freetext" href="http://www.sciencemag.org/cgi/data/1142538/DC1/1">http://www.sciencemag.org/cgi/data/1142538/DC1/1</a><br>
<br>
Best regards,<br>
<br>
- Jon<br>
<br>
Jon D. Moulton, Ph.D.<br>
Diagnostics and Special Projects<br>
GENE TOOLS, LLC<br>
<a class="moz-txt-link-abbreviated" href="mailto:jmoulton@gene-tools.com">jmoulton@gene-tools.com</a><br>
<a class="moz-txt-link-freetext" href="http://network.nature.com/group/morpholinos">http://network.nature.com/group/morpholinos</a><br>
<br>
<a class="moz-txt-link-abbreviated" href="mailto:yang.x@neu.edu">yang.x@neu.edu</a> wrote:
<blockquote
cite="mid:c061b015-a02b-451a-ba55-3efb44888ad3@c65g2000hsa.googlegroups.com"
type="cite">
<pre wrap="">Hi, I just started to do microinjection. I am wondering how to
calibrate the needles so that I know the amount I am injecting. Thanks
a lot!
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