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<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "> Jocelyn,<div><br class="webkit-block-placeholder"></div><div>I have not used Gibco's hanks for IVF in zebrafish but many short term extenders used in other fish species include glucose. I don't think the slightly higher osmolarity will be a problem since high osmolarity sequesters sperm activation.</div><div><br class="webkit-block-placeholder"></div><div>I'd give it a shot.</div><div><br class="webkit-block-placeholder"></div><div><br class="webkit-block-placeholder"></div><div>Good luck,</div><div><br class="webkit-block-placeholder"></div><div>Dan</div><div><br class="webkit-block-placeholder"></div><div><br></div><div><br><div> <span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div>Daniel Castranova <br>Aquatic Specialist <br>Charles River Laboratories - Contractor <br>Unit on Vertebrate Organogenesis <br>Laboratory of Molecular Genetics, NICHD, NIH <br>Building 6B, Room 322, 6 Center Drive <br>Bethesda, MD 20892 <br>(301)594-0904 <br><a href="mailto:castrand@mail.nih.gov">castrand@mail.nih.gov</a></div><br class="Apple-interchange-newline"></span></span> </div><br><div><div>On Aug 12, 2008, at 2:21 PM, Jocelyn wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Has anyone used Gibco's Hanks in an invitro fertilization protocol?</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">I'm noticing that Gibco's formula includes glucose (to up the</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">osmolarity?), where as the Zebrafish book recipe does not use glucose.</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Will this affect the viability of the sperm?</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">I need to do a squeeze, and don't have the stock solution components</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">on hand to make hanks, but instead have the Gibco premade 10x hanks (-</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">bicarb, - phenol red).</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Any advice?</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">_______________________________________________</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Zbrafish mailing list</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="mailto:Zbrafish@net.bio.net">Zbrafish@net.bio.net</a></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.bio.net/biomail/listinfo/zbrafish">http://www.bio.net/biomail/listinfo/zbrafish</a></div> </blockquote></div><br></div></body></html>

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