<span style="border-collapse:collapse;font-size:11px"><span style="font-size:large">We have purchased four different types of microinjection molds from Adaptive Science Tools at <a href="http://adaptivesciencetools.com/index.html" style="color:rgb(42, 93, 176)" target="_blank">http://adaptivesciencetools.com/index.html</a> and use them according to what we are doing with the embryos. <span>All types of molds are $25.00 each. </span></span><div>
<span style="font-size:17px"><br></span><div><div><span style="font-size:17px">Zebrafish Injection:</span></div><div><span style="font-size:17px"><span style="font-size:14px"><span style="font-weight:bold">TU-1</span> = Six ramps containing one 90 degree and one 45 degree beveled side.</span></span></div>
<div><span style="font-size:14px"><br></span></div><div><span style="font-size:17px"><span style="font-size:14px"><span style="font-weight:bold">Cnf-2</span> = Two ramps for holding dechorioned embryos used for time-lapse dorsal or ventral imaging</span></span></div>
<div><span style="font-size:14px"><br></span></div><div><span><span style="font-size:large">Cell transplantation</span></span><span style="font-size:14px">:</span></div><div><span><span style="font-size:large"><span style="font-weight:bold">PT-1</span></span></span><span style="font-size:14px">= Injection/ transplantation mold with 150 triangular divots to accept individual embryos. <br>
</span></div><div><span style="font-size:14px"><br></span></div><div><span style="font-size:14px"><span style="font-weight:bold">I-34 = </span>Six ramps used for micro-injection. Both sides are at a 90-degree angle firmly orienting the embryo.<br>
</span></div></div><div><span style="font-size:14px"><br></span><div><span style="font-size:14px"><br></span></div><div><span style="font-size:17px"><span style="font-size:14px"><span style="font-size:17px">To order molds call (774) 239-6133 or email Inject@AdaptiveScienceTools.com</span></span></span></div>
<div><span style="font-size:17px"><br></span></div><div><span style="font-size:17px"><span style="font-size:14px"><span style="font-size:17px">They will also make molds and other bench top and fish facility equipment to your specifications. See the website for more details.</span></span></span></div>
<div><span style="font-size:17px"><br></span></div><div><span style="font-size:17px"><span style="font-size:14px"><span style="font-size:17px">Hope this helps.</span></span></span></div><div><span style="font-size:17px"><br>
</span><br><div class="gmail_quote">On Fri, Jan 30, 2009 at 1:23 PM, Cecilia Moens <span dir="ltr"><<a href="mailto:cmoens@fhcrc.org" style="color:rgb(42, 93, 176)" target="_blank">cmoens@fhcrc.org</a>></span> wrote:<br>
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<div style="word-wrap:break-word"><div>We purchased our microinjection molds from Clive Ellard Instrumentation in Washington (360) 805-5406, <a href="mailto:clive@ellardinstrumentation.com" style="color:rgb(42, 93, 176)" target="_blank">clive@ellardinstrumentation.com</a>. He sells a mold for making agarose dishes with six parallel troughs for microinjection, for $40.00 each. I'm not sure what the part number is, but tell them you want the mold they made for the Moens lab at the Fred Hutchinson Cancer Research Center. Also, Adaptive Science Tools in MA (774) 239-6133 sells a plastic mold for making agarose dishes with 120 single wells for individual embryos (part # PT-1). These are useful for transplantation experiments. They may sell a mold for making troughs like the one from Clive Ellard, but I'm not certain about this. </div>
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