<span class="Apple-style-span" style="font-size: large;">We have purchased four different types of microinjection molds from Adaptive Science Tools at <a href="http://adaptivesciencetools.com/index.html">http://adaptivesciencetools.com/index.html</a> and use them according to what we are doing with the embryos. <span class="Apple-style-span" style="-webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px; ">All types of molds are $25.00 each. </span></span><div>
<span class="Apple-style-span" style="font-size: 17px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><br></span><div><div><span class="Apple-style-span" style="font-size: 17px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;">Zebrafish Injection:</span></div>
<div><span class="Apple-style-span" style="font-size: 17px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><span class="Apple-style-span" style="font-size: 14px; "><span class="Apple-style-span" style="font-weight: bold;">TU-1</span> = Six ramps containing one 90 degree and one 45 degree beveled side.</span></span></div>
<div><span class="Apple-style-span" style="font-size: 14px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><br></span></div><div><span class="Apple-style-span" style="font-size: 17px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><span class="Apple-style-span" style="font-size: 14px; "><span class="Apple-style-span" style="font-weight: bold;">Cnf-2</span> = Two ramps for holding dechorioned embryos used for time-lapse dorsal or ventral imaging</span></span></div>
<div><span class="Apple-style-span" style="font-size: 14px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><br></span></div><div><span class="Apple-style-span" style="-webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px; "><span class="Apple-style-span" style="font-size: large;">Cell transplantation</span></span><span class="Apple-style-span" style="font-size: 14px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;">:</span></div>
<div><span class="Apple-style-span" style="-webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px; "><span class="Apple-style-span" style="font-size: large;"><span class="Apple-style-span" style="font-weight: bold;">PT-1</span></span></span><span class="Apple-style-span" style="font-size: 14px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;">= Injection/ transplantation mold with 150 triangular divots to accept individual embryos. <br>
</span></div><div><span class="Apple-style-span" style="font-size: 14px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><br></span></div><div><span class="Apple-style-span" style="font-size: 14px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><span class="Apple-style-span" style="font-weight: bold;">I-34 = </span>Six ramps used for micro-injection. Both sides are at a 90-degree angle firmly orienting the embryo.<br>
</span></div></div><div><span class="Apple-style-span" style="font-size: 14px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><br></span><div><span class="Apple-style-span" style="font-size: 14px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><br>
</span></div><div><span class="Apple-style-span" style="font-size: 17px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><span class="Apple-style-span" style="font-size: 14px;"><span class="Apple-style-span" style="font-size: 17px; ">To order molds call (774) 239-6133 or email Inject@AdaptiveScienceTools.com</span></span></span></div>
<div><span class="Apple-style-span" style="font-size: 17px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><br></span></div><div><span class="Apple-style-span" style="font-size: 17px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><span class="Apple-style-span" style="font-size: 14px;"><span class="Apple-style-span" style="font-size: 17px;">They will also make molds and other bench top and fish facility equipment to your specifications. See the website for more details.</span></span></span></div>
<div><span class="Apple-style-span" style="font-size: 17px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><br></span></div><div><span class="Apple-style-span" style="font-size: 17px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><span class="Apple-style-span" style="font-size: 14px;"><span class="Apple-style-span" style="font-size: 17px;">Hope this helps.</span></span></span></div>
<div><span class="Apple-style-span" style="font-size: 17px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><br></span></div><div><span class="Apple-style-span" style="font-size: 17px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><span class="Apple-style-span" style="font-size: 14px;"><span class="Apple-style-span" style="font-size: 17px;">-Elizabeth<br>
</span></span></span><div><span class="Apple-style-span" style="font-size: 14px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><br></span></div><div><span class="Apple-style-span" style="font-size: 14px; -webkit-border-horizontal-spacing: 5px; -webkit-border-vertical-spacing: 5px;"><br>
</span><br><div class="gmail_quote">On Fri, Jan 30, 2009 at 1:23 PM, Cecilia Moens <span dir="ltr"><<a href="mailto:cmoens@fhcrc.org">cmoens@fhcrc.org</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex;">
<div style="word-wrap:break-word"><div>We purchased our microinjection molds from Clive Ellard Instrumentation in Washington (360) 805-5406, <a href="mailto:clive@ellardinstrumentation.com" target="_blank">clive@ellardinstrumentation.com</a>. He sells a mold for making agarose dishes with six parallel troughs for microinjection, for $40.00 each. I'm not sure what the part number is, but tell them you want the mold they made for the Moens lab at the Fred Hutchinson Cancer Research Center. Also, Adaptive Science Tools in MA (774) 239-6133 sells a plastic mold for making agarose dishes with 120 single wells for individual embryos (part # PT-1). These are useful for transplantation experiments. They may sell a mold for making troughs like the one from Clive Ellard, but I'm not certain about this. </div>
<div><br></div><div>- Cecilia.</div><br><div> <span style="border-collapse:separate;color:rgb(0, 0, 0);font-family:Helvetica;font-size:12px;font-style:normal;font-variant:normal;font-weight:normal;letter-spacing:normal;line-height:normal;text-align:auto;text-indent:0px;text-transform:none;white-space:normal;word-spacing:0px"><div style="word-wrap:break-word">
<p style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px"><font face="Arial" size="2">Cecilia B. Moens</font></p><p style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px"><font face="Arial" size="2">Division of Basic Science</font></p>
<p style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px"><font face="Arial" size="2">Fred Hutchinson Cancer Research Center</font></p><p style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px">
<font face="Arial" size="2">B2-152, 1100 Fairview Ave. N.<span> </span></font></p><p style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px"><font face="Arial" size="2">Seattle, WA 98109-1024</font></p><p style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;min-height:11px">
<br></p><p style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px"><font face="Arial" size="2">office: (206) 667-5627</font></p><p style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px">
<font face="Arial" size="2">lab: (206) 667-5697</font></p><p style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px"><font face="Arial" size="2">fax: (206) 667-3308</font></p></div></span> </div><br></div>
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