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Hi Tess,<div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 12px/normal Verdana; min-height: 15px; ">Here is my recommendation for containing Coleps and/or any other unwanted organisms.</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 12px/normal Verdana; min-height: 15px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Verdana" size="3" style="font: 12.0px Verdana">1. Bleach all of your eggs as soon as possible. If needed, you can reference the egg bleaching protocol found in The Zebrafish Book, <a href="http://zfin.org/zf_info/zfbook/chapt3/3.2.html">http://zfin.org/zf_info/zfbook/chapt3/3.2.html</a> or on our website, <a href="http://zebrafish.org/zirc/documents/protocols.php#Egg%20Bleaching">http://zebrafish.org/zirc/documents/protocols.php#Egg%20Bleaching</a>.</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Verdana" size="3" style="font: 12.0px Verdana">2. Be sure to remove ALL dead/damaged eggs before bleaching.</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Verdana" size="3" style="font: 12.0px Verdana">3. After bleaching, keep your embryos in embryo media until they have inflated their swim bladders and are swimming. Zebrafish larvae are the most susceptible when they have hatched, but not yet inflated their swim bladders. Once the larvae are able to swim properly, they can fend off most organisms.</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 12px/normal Verdana; min-height: 15px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 12px/normal Verdana; min-height: 15px; ">If you haven't already positively identified the organisms and would like to, I recommend putting a few of these critters on a slide and look at them under a compound microscope. Coleps are quite small and move in an intense twirling motion. They have plates that cover their exterior and make them look like hand grenades. As you mentioned, they are voracious and can consume an entire embryo in 45 minutes. </div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Verdana" size="3" style="font: 12.0px Verdana">For future reference, I recommend obtaining a good protozoa picture book/guide. We have 2 older books that are quite helpful, however you might be able to find some newer better books with even better pictures and information.</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; font: normal normal normal 12px/normal Verdana; min-height: 15px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Verdana" size="3" style="font: 12.0px Verdana">- Patterson, D.J. (1996). Free-Living Freshwater Protozoa (A Colour Guide). John Wiley & Sons Inc., New York, New York.</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Verdana" size="3" style="font: 12.0px Verdana">- Berk, S. G.; Gunderson, J. H (1993). Wastewater Organisms (A Color Atlas). CRC Press LLC. </font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">If you continue to have problems, please let me know.</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">Very best,</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><br></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">April</div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><br></div><div><div>On Feb 9, 2009, at 4:17 PM, Fresquez, Theresa, DCA wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"> <div><font face="Arial" color="#000000" size="2">Hello,</font></div> <div><font face="Arial" size="2">Does anyone know how to get rid of coleps? They are eating my zebrafish babies. </font></div> <div><font face="Arial" size="2">Thanks,</font></div> <div><font face="Arial" size="2">Tess</font></div> <div><font face="Arial" size="2"></font> </div> <div><a class="divRcpUnresCIn" onclick="return(false);" href="" name="unresolved" nindex="0"><font color="#ff0000"></font></a> </div><div> </div><br><br>Confidentiality Notice: This e-mail, including all attachments is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. 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