<div>Dear dr. Vayuputra,</div>
<div> </div>
<div>I came across your question regarding combined in situ hybridisation using anti-DIG-rhodamin as a detection method for your hybridisation, combined with fluorescent immunohistochemistry.</div>
<div>I am trying the same now and encounter the same problems (no detection), while detection with anti-DIG AP and subsequent NBT-BCIP precipitation yields nice results. Have you succeeded in visualizing your probe fluorescently? If yes, I'd very much appreciate your advice.</div>
<div> </div>
<div>Thank you very much in advance,<br clear="all"><br>-- <br>Ming-San Ma, MD<br>Dept. of Neuroscience<br>University Medical Center Groningen<br>9713 AV Groningen, The Netherlands<br>+31-50-3632759<br><a href="mailto:mingsanma@gmail.com">mingsanma@gmail.com</a><br>
<a href="http://www.mingsanma.com">www.mingsanma.com</a> (under construction)<br></div>
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