<DIV>Re: Morpholino dose--ng or pg per embryo (Chao Zhang)</DIV>
<DIV> </DIV>
<DIV>Hi Zhang Chao,</DIV>
<DIV>How are you? You did wrong calculation, 0.5mM * 1nL * 8300g/mol should be 4.15 ng. Please recalculate. </DIV>
<DIV>Best</DIV>
<DIV>Huiqing</DIV>
<DIV><BR><BR>----- Original Message -----<BR>From: zbrafish-request@oat.bio.indiana.edu<BR>Date: Saturday, March 28, 2009 1:03 pm<BR>Subject: Zbrafish Digest, Vol 46, Issue 12<BR>To: zbrafish@magpie.bio.indiana.edu<BR><BR>> Send Zbrafish mailing list submissions to<BR>> zbrafish@net.bio.net<BR>> <BR>> To subscribe or unsubscribe via the World Wide Web, visit<BR>> http://www.bio.net/biomail/listinfo/zbrafish<BR>> or, via email, send a message with subject or body 'help' to<BR>> zbrafish-request@net.bio.net<BR>> <BR>> You can reach the person managing the list at<BR>> zbrafish-owner@net.bio.net<BR>> <BR>> When replying, please edit your Subject line so it is more specific<BR>> than "Re: Contents of Zbrafish digest..."<BR>> <BR>> <BR>> Today's Topics:<BR>> <BR>> 1. Morpholino dose--ng or pg per embryo (Chao Zhang)<BR>> <BR>> <BR>> -----------------------------------------------------------------<BR>> -----<BR>> <BR>> Message: 1<BR>> Date: Fri, 27 Mar 2009 14:31:57 -0700<BR>> From: "Chao Zhang" <zhangch@ohsu.edu><BR>> Subject: [Zbrafish] Morpholino dose--ng or pg per embryo<BR>> To: <zbrafish@magpie.bio.indiana.edu><BR>> Message-ID: <F58AB4839D379649A9E190A61925764A02044E@EX-<BR>> BE06.ohsu.edu>Content-Type: text/plain; charset="gb2312"<BR>> <BR>> Hi:<BR>> I have a Morpholino oligo at 8300g/mol.<BR>> Working solution is 0.5mM, 1nL is injected into each embryo.<BR>> That should be 0.5mM * 1nL * 8300g/mol=4.15* 10E-12 <BR>> g/embryo(4.15pg/embryo), right?<BR>> Why zebrafish guys always say 4* 10E-9 g/embryo(4ng/embryo)?<BR>> Anybody could give me an answer? <BR>> Thanks.<BR>> -------------- next part --------------<BR>> An HTML attachment was scrubbed...<BR>> URL: <BR>> http://www.bio.net/bionet/mm/zbrafish/attachments/20090327/206c8e85/attachment-0001.html<BR>> <BR>> ------------------------------<BR>> <BR>> _______________________________________________<BR>> Zbrafish mailing list<BR>> Zbrafish@net.bio.net<BR>> http://www.bio.net/biomail/listinfo/zbrafish<BR>> <BR>> End of Zbrafish Digest, Vol 46, Issue 12<BR>> ****************************************<BR>> </DIV><BR><BR>Huiqing Li
<br>Ph.D Candidate
<br>Center of Marine Biotechnology
<br>701 E Pratt St.
<br>Baltimore, MD 21202</BR></BR>
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