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Hi all, <br>
<br>
since a few weeks, some PhD students in our lab have got problems with
their wild type, non injected embyos: <br>
- some have a delay in development (14 somites instead of 24); <br>
- in some dishes, there are 3 or more different developmental stages at
24hpf (while the eggs were collected at the same time, with a 15
minutes interval between each clutch); <br>
- some clutches show >50 % mortality at 24hpf. <br>
We also have some pairs laying only dead eggs. <br>
<br>
This occurs in 25-30% of the clutches. At this time, the transgenics
eggs don't seem to be affected. It seems that this occurs more
frequently with eggs collected in the
afternoon. The parent fish are 9-12 months old. Recently, there has
been some NH4+/NH3 and NO2 in the system, but now it's decreased.<br>
<br>
We pay attention to let only 50 eggs in the 8cm petri dishes, change
water at least once a day, discard unfertilised eggs at 4hpf. <br>
<br>
Have you any idea of the possible cause(s) of this? Do you think that
the N ions could have influenced individuals at different levels,
making some of them produce good eggs and other no? <br>
<br>
Thank you in advance for any shared idea and help. <br>
<br>
best regards, <br>
<br>
Marie<br>
<br>
<br>
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